Ontology highlight
ABSTRACT:
PROVIDER: PRJDB20317 | ENA |
REPOSITORIES: ENA
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2017-03-23 | GSE94060 | GEO
Project description:Pseudouridine is an isomer of uridine and is the most common RNA modification in both procaryotes and eucaryotes. It is found in ribosomal, transfer, and other structural RNA as well as in some mRNA and non-coding RNA. We have found abundant pseudouridine in small RNA and their precursors in Arabidopsis. This work was supported by the Howard Hughes Medical Institute and by grants from the National Institutes of Health (R35GM144206), the National Science Foundation Plant Genome Research Program and the Robertson Research Foundation (to R.A.M.). Work in the Kouzarides laboratory is supported by a grant from Cancer Research UK (grant no. RG96894), in addition to benefiting from core support from the Wellcome Trust (WT203144) and Cancer Research UK (grant no. C6946/A24843). V.M. was funded by a Kay Kendall Leukemia Fund project grant (grant no. RG88664) and Cancer Research UK (grant no. RG96894). J.D. was funded by a grant from the Polish National Science Center (2020/39/D/NZ1/01918). We acknowledge assistance from the Cold Spring Harbor Laboratory Shared Resources, which are funded in part by the Cancer Center support grant (5PP30CA045508)
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Project description:We propose a novel approach for FPOP data analysis, utilizing DIA data. The HbHp protein complex was analyzed by FPOP and measured on timsToF SCP in DIA, DDA and MS modes. The IDs of modified peptides were quantified for each acquisition mode and the extent of modification was calculated on the level of peptides. The reproducibility was evaluated by coefficients of variation.This work was mainly financially supported by the Czech Science Foundation (22-27695S), the Technology Agency of the Czech Republic (ODEEP-EU TH86010001), the Ministry of Education, Youth and Sports of the Czech Republic grant PHOTOMACHINES - Photosynthetic cell redesign for high yields of therapeutic peptides (CZ.02.01.01/00/22_008/0004624) and the Academy of Sciences of the Czech Republic (RVO: 61388971).
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Project description:Microarrays were used to identify transcriptional responses in field-grown root material of wheat in order to dissect specific gene expression responses to limited macronutrient availability, particularly phosphate. This study fills the gap between the transcriptome studies on model plants and the lack of studies on soil-grown wheat aiming to identify candidate genes for enhancing nutrient uptake efficiency. The work at Rothamsted Research is supported via the 20:20 Wheat® Programme by the UK Biotechnology and Biological Sciences Research Council. The contribution was supported by BIONUT-ITN and the research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 264296.
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