ABSTRACT: Japan Antimicrobial Resistant Bacterial Surveillance of Gram-negative Rods (JARBS-GNR) and Vancomycin-Resistant Enterococci (JARBS-VRE): surveillance between 2019 and 2020
Project description:This study aims to determine the global gene expression in vancomycin resistant Enterococcus faecium (VRE) in response to a novel essential oil-vancomycin combination, and the individual components (vancomycin, carvacrol and cuminaldehyde) to help determine the mechanism of action of this antimicrobial formulation. This formulation increases the susceptibility of VRE to vancomycin and the array provides data on the synergistic mechanism of action. Five conditions (1. Control; 2. Carvacrol, 1.98 mM; 3. Cuminaldehyde, 4.20 mM; 4. Vancomycin, 0.031 mg/l; 5. Combination, 1.98 mM Carvacrol, 4.2 mM Cuminaldehyde, 0.031 mg/l vancomycin) all with 1% DMSO were tested in triplicate with a 60 minute exposure time before extraction.
Project description:In order to determine the mechanism of Cajanin Stilbene Acid inhibiting vancomycin-resistant enterococci, we compared the changes in protein expression of enterococci V583 strain before and after treated by Cajanin Stilbene Acid.
