ABSTRACT: 16S rRNA gene fragment sequences from surface seawater collected in Nov 2011 at coastal area of Noto town (37˚18’ N, 137˚14’ E, depth of 0 m).
Project description:16S rRNA gene fragment sequences from mesopelagic seawater collected in Nov 2011 at coastal area of Noto town, Ishikawa, Japan (37˚17’ N, 137˚16’ E, depth of 320 m).
Project description:16S rRNA gene fragment sequences from surface seawater collected in Nov 2011 at coastal area of Muroto city, Kochi, Japan (33˚18’ N, 134˚11’ E, depth of 0.5 m).
Project description:16S rRNA gene fragment sequences from mesopelagic seawater collected in Nov 2011 at coastal area of Suruga bay, Shizuoka, Japan (34˚51’ N, 138˚21’ E, depth of 397 m).
Project description:16S rRNA gene fragment sequences from surface seawater collected in July 2011 at coastal area of Muroto city, Kochi, Japan (33˚18’ N, 134˚11’ E, depth of 0.5 m).
Project description:16S rRNA gene fragment sequences from Mesopelagic seawater collected in July 2011 at coastal area of Muroto city, Kochi, Japan (33˚18’ N, 134˚14’ E, depth of 320 m)
Project description:Interventions: "A group: MC followed by EUS" for diagnosis of invasion depth From February 2011 to December 2013
"B group: EUS followed by MC" for diagnosis of invasion depth From February 2011 to December 2013
Primary outcome(s): Accuracy rate for invasion depth of early colorectal cancer by MC and EUS
Study Design: Cross-over Randomized
Project description:Using a combination of cell sorting and microarray analysis, we identified almost 200 genes as having a high level of expression in the notochord. After whole mount in situ hybridization screening, we confirmed approximately one third of these as having a novel notochord expression pattern. Keywords: cell type comparison - embryonic Noto-GFP+ notochord progenitors versus surrounding GFP- comparator cells 3 biological replicates - each replicate includes an experiment cell population (Noto-GFP positive cell sort) and a comparator population (GFP negative cell sort)
Project description:Formation of motile cilia in vertebrate embryos is essential for proper development and tissue function. Key regulators of motile ciliogenesis are the transcription factors FOXJ1 and NOTO, which are conserved throughout vertebrates. Downstream target genes of FOXJ1 have been identified in a variety of species, organs and cultured cell lines; in murine embryonic and foetal tissues, however, FOXJ1 and NOTO effectors have not been comprehensively analysed and our knowledge of the downstream genetic programme driving motile ciliogenesis in the mammalian lung and ventral node is fragmentary. We compared genome-wide expression profiles of undifferentiated E14.5 vs. abundantly ciliated E18.5 micro-dissected airway epithelia as well as Foxj1+ vs. Foxj1-deficient foetal (E16.5) lungs of the mouse using microarray hybridisation. 326 genes deregulated in both screens are candidates for FOXJ1-dependent, ciliogenesis-associated factors at the endogenous onset of motile ciliogenesis in the lung, including 123 genes that have not been linked to ciliogenesis before; 46% of these novel factors lack known homologues outside mammals. Microarray screening of Noto+ vs. Noto null early headfold embryos (E7.75) identified 59 of the lung candidates as NOTO/FOXJ1-dependent factors in the embryonic left-right organiser that carries a different subtype of motile cilia. For several uncharacterised factors from this small overlap – including 1700012B09Rik, 1700026L06Rik and Fam183b – we provide extended experimental evidence for a ciliary function.
