Project description:Genomic DNA extracted from IMR-90 cells were subjected to library preparations with tPBAT and sPBAT protocols. Sequencing was done with HiSeq X Ten platform and reads obtained were mapped with our own pipeline. The methylome data produced were compared. There is a little differences between the two datasets, which indicate sPBAT developped here is comparable with tPBAT protocol.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
