Project description:To determine whether target genes were specifically knocked down by RfxCas13d in N2a cells, we performed RNA-seq to compare the differentially expressed genes between cells transfected with targeting crRNAs and non-targeting crRNA.
Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.
2020-08-10 | GSE60694 | GEO
Project description:PolyA-seq analysis of Fus/U1 snRNP-kdepleted N2A cells
Project description:FUS is an RNA binding protein that plays an important role in various cellular processes including RNA splicing, DNA repair and transcriptional regulation. However, the RNA binding capacity of FUS in atherosclerosis is unclear.we knocked down FUS with siRNA to further study the overall transcriptional level and select alternative splicing (AS) of FUS regulation in human umbilical vein endothelial cells (HUVECs) by RNA sequencing. The qRT-PCR strategy was used to validate FUS-modulated genes.Knockdown of FUS had significant effects on gene expression in HUVECs. Knock-down of FUS resulted in 200 differentially expressed genes (DEGs) that were highly related to apoptotic process, signal transduction, multicellular organism development, cell adhesion and regulation of transcription, DNA-templated pathways. Importantly, FUS extensively regulated 2870 alternative splicing events with a significant difference. Functional analysis of its-modulated alternative splicing genes revealed they were highly enriched in cell cycle, cell population proliferation and G2/M transition of mitotic cell cycle pathways. The results of qRT-PCR were consistent with the RNA-seq analysis.
2025-05-19 | GSE203426 | GEO
Project description:RNA-seq of Car4 knocked-down cells