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Nasutitermes takasagoensis

ABSTRACT: cDNA library sequencing in the midgut, mixed segment and first proctodeal segment of worker termites in Nasutitermes takasagoensis by 454 GS Junior pyrosequencing

PROVIDER: PRJDB3481 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR029660.fastq.gz	Fastqsanger.gz
	DRR029661.fastq.gz	Fastqsanger.gz
	DRR029662.fastq.gz	Fastqsanger.gz
	DRR050638.fastq.gz	Fastqsanger.gz
	DRR050639.fastq.gz	Fastqsanger.gz

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Nasutitermes takasagoensis

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Project description:Complete genomes reconstructed with high-fidelity long reads metagenomic from the gut of Nasutitermes takasagoensis

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Localization of symbiotic clostridia in the mixed segment of the termite Nasutitermes takasagoensis (Shiraki).

Project description:Phylogeny and the distribution of symbiotic bacteria in the mixed segment of the wood-eating termite Nasutitermes takasagoensis (Shiraki) were studied. Bacterial 16S rRNA genes (rDNA) were amplified from the mixed segment of the gut by PCR, and two kinds of sequences were identified. The phylogenetic tree was constructed by neighbor-joining and maximum parsimony methods to identify symbionts harbored in the mixed segment. They are classified as low-G+C-content gram-positive bacteria and are most closely related to the genus Clostridium. The distribution of these bacteria throughout the whole gut was examined by PCR using specific primers, which suggested that they are confined to the mixed segment despite the presence of bacteria throughout the gut. In situ hybridization indicated that the symbiotic bacteria were localized to the ectoperitrophic space between the midgut wall and the peritrophic membrane in the mixed segment. Electron microscopy revealed the close association between these bacteria and the mesenteric epithelium, suggesting that they have some interactions with the gut tissue of termites.

| S-EPMC101473 | biostudies-literature

Manduca sexta differential gene expression, male & female antenna, larval midgut

Project description:To allow estimation of the complexity of the antennal transcriptome between sexes as well as in comparison with a non-antennal tissue, microarrays were designed based on an assembly of new 454 sequence data from the antenna and publicly available data including 454 data from the larval midgut. The microarrays were hybridized with samples generated from the repsecitve tissues from 3 (antenna) and 5 (midgut) animals per sample, with four independent samples per sex (antenna) and tissue (midgut).

2011-03-01 | GSE27470 | GEO

termite gut metagenome

Project description:Nasutitermes corniger midgut segment microbial community from laboratory colony in Florida, USA - Nc150M

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Heterologous expression in Pichia pastoris and characterization of an endogenous thermostable and high-glucose-tolerant β-glucosidase from the termite Nasutitermes takasagoensis.

Project description:Termites are well-known cellulose decomposers and can give researchers insights into how to utilize lignocellulosic biomass in the actual scenario of energy consumption. In this work, an endogenous β-glucosidase from the midgut of the higher termite Nasutitermes takasagoensis was purified to homogeneity by Ni(2+) affinity chromatography and its properties were characterized. This β-glucosidase (G1mgNtBG1), which belongs to glycoside hydrolase family 1, is a homotrimer in its native form, with a molecular mass of 169.5 kDa, as demonstrated by gel filtration chromatography. The enzyme displayed maximum activity at pH 5.5 and had broad substrate specificities toward several saccharides, including cellobiose. G1mgNtBG1 showed a relatively high temperature optimum of 65°C and one of the highest levels of glucose tolerance among several β-glucosidases already characterized, with a K(i) of 600 mM glucose. To examine the applicability of G1mgNtBG1 in biomass conversion, we compared the thermostability and glucose tolerance of G1mgNtBG1 with those of Novozym 188. We found that G1mgNtBG1 was more thermostable after 5 h of incubation at 60°C and more resistant to glucose inhibition than Novozym 188. Furthermore, our result suggests that G1mgNtBG1 acts synergistically with Celluclast 1.5 L in releasing reducing sugars from Avicel. Thus, G1mgNtBG1 seems to be a potential candidate for use as a supplement in the hydrolysis of biomass.

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Project description:Four cDNA libraries from immature embryos, mature embryos, microspore derived embryos and mature leaves were constructed. cDNA were sequenced by the Roche-454 GS-FLX.

2017-01-11 | E-MTAB-3226 | biostudies-arrayexpress

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