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Bioreactor metagenome

ABSTRACT: Optimal phosphate concentration for growth and normal functioning of the marine anammox bacteria, Candidatus Scalindua sp.

PROVIDER: PRJDB35461 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR698126_1.fastq.gz	Fastqsanger.gz
	DRR698126_2.fastq.gz	Fastqsanger.gz
	DRR698127_1.fastq.gz	Fastqsanger.gz
	DRR698127_2.fastq.gz	Fastqsanger.gz
	DRR698128_1.fastq.gz	Fastqsanger.gz

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Transcriptome Kuenenia stuttgartiensis bioreactor

Project description:Anaerobic ammonium-oxidising (anammox) bacteria, members of the ‘Candidatus Brocadiaceae’ family, play an important role in the nitrogen cycle and are estimated to be responsible for about half of the oceanic nitrogen loss to the atmosphere. Anammox bacteria combine ammonium with nitrite and produce dinitrogen gas via the intermediates nitric oxide and hydrazine (anammox reaction) while nitrate is formed as a by-product. These reactions take place in a specialized, membrane-bound compartment called the anammoxosome. Therefore, the substrates ammonium, nitrite and product nitrate have to cross the outer-, cytoplasmic- and anammoxosome membranes to enter or exit the anammoxosome. The genomes of all anammox species harbour multiple copies of ammonium-, nitrite- and nitrate transporter genes. Here we investigated how the distinct genes for ammonium-, nitrite- and nitrate- transport were expressed during substrate limitation in membrane bioreactors. Transcriptome analysis of Kuenenia stuttgartiensis planktonic cells under ammonium-limitation showed that three of the seven ammonium transporter genes and one of the six nitrite transporter genes were significantly upregulated, while another ammonium and nitrite transporter gene were downregulated in nitrite limited growth conditions. The two nitrate transporters were expressed to similar levels in both conditions. In addition, genes encoding enzymes involved in the anammox reaction were differentially expressed, with those using nitrite as a substrate being upregulated under nitrite limited growth and those using ammonium as a substrate being upregulated during ammonium limitation. Taken together, these results give a first insight in the potential role of the multiple nutrient transporters in regulating transport of substrates and products in and out of the compartmentalized anammox cell.

2020-06-05 | GSE148825 | GEO

bioreactor metagenome

Project description:Genome-based Metagenomic Analysis of Candidatus Scalindua with Anammox consortia in Long-term Cultivated Marine Anammox Bacteria Community

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Pilot project to determine optimal bioreactor protein glycosylation conditions

2011-04-29 | GSE28920 | GEO

bioreactor metagenome

Project description:Bioreactor Anammox bacterial community from Nijmegen, The Netherlands - Scalindua species enirchment metagenome

| PRJNA337667 | ENA

Proteome of "Candidatus Kuenenia Stuttgartiensis" strain CSTR1 growing planctonically in semi-CSTR reactor

Project description:The dataset provides the whole proteome of the anammox bacterium "Candidatus Kuenenia Stuttgartiensis" strain CSTR1 growing planctonically in semi-CSTR reactor. The bacteria were growing at high growth rate (0.33 d-1) (reactor HRT 3d).

2020-11-11 | PXD018553 | Pride

Pilot project to determine optimal bioreactor protein glycosylation conditions

Project description:These research areas concentrate on stress induced proteases in recombinant Escherichia coli, glycosylation heterogeneity due to bioprocess conditions produced in mammalian cells, and metabolic engineering of E. coli. The hypothesis of this project is that recombinant protein glycosylation is inefficient under normal bioreactor conditions since the additional glycosylation reactions necessary for the recombinant protein exceed the metabolic capacity of the cells. Normal bioreactor conditions have been optimized for cell growth, and sometimes for protein productivity. Only recently has it been accepted that optimal glycosylation may not occur under optimal growth or protein productivity conditions. Specific Aim: Determine the relationship between bioreactor conditions and glycosylation gene expression in NS0 cells. EXPERIMENT: Mouse NS0 myeloma cells were grown in culture, stressed with 5 mM NaCl, 10 mM proline plus 5 mM ammonia, or 5 mM ammonia, along with an unstressed control group. The growth of the cultures were followed until the late exponential phase (90 hours), at which time two 50 mLs of cells were harvested and RNA extracted. Samples were prepared in triplicate, for a total of 12 samples. The RNA was amplified and labeled by Microarray Core (E) and hybridized to the GLYCOv3 microarrays.

2011-04-29 | E-GEOD-28920 | biostudies-arrayexpress

Candidatus Scalindua japonica

Project description:Genome sequence of an marine anammox bacterium, Candidatus "Scalindua japonica"

| PRJDB740 | ENA

Candidatus Scalindua

Project description:Candidatus Scalindua sp. overview

| PRJNA266259 | ENA

bioreactor metagenome

Project description:Reactor performance and microbial community structure of single-stage partial nitritation anammox membrane bioreactors inoculated with Brocadia and Scalindua enrichment cultures

| PRJDB10672 | ENA

Candidatus Scalindua sp.

Project description:Candidatus Scalindua sp. Raw sequence reads

| PRJNA1035435 | ENA