Project description:Comparative gene expression profiling analysis of RNA-seq data from six venom-gland samples belonging to the Ag and Ng groups. The transcriptome data of the venom gland of P. lewisi obtained in this study provide a theoretical foundation for identifying the components and studying the biological functions of P. lewisi venom.
Project description:Diachasmimorpha longicaudata parasitoid wasps carry a symbiotic poxvirus, known as DlEPV, within the female wasp venom gland. We sequenced RNA from venom gland tissue to identify DlEPV orthologs for 3 conserved poxvirus core genes. The DlEPV ORFs identified from this transcriptome were used to design primers for downstream RT-qPCR analysis and RNAi knockdown experiments.
