Project description:Transcriptional profiling of gamma-oryzanol-treated (24h) differentiating equine satellite cells (3rd day of differentiation) compared to control GO-untreated cells. Goal was to determine the effects of gamma-oryzanol influence on gene expression in equine satellite cells during in vitro myogenesis Two-condition experiment, GO-treated (24h) differentiating equine satellite cells (3rd day of differentiation) vs. differentiating equine satellite cells without GO treatment (control). Biological replicates: 4 reps of examined condition (Cy5), 4 control replicates (Cy3)
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to GO and rGO on nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments using age synchronized young adult C. elegans population exposed to GO and rGO for 24h. We used whole genome microarrays to screen for global changed in C. elegans transcription profiles and with subsequent quantitative analysis conducted on selected genes.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to GO and rGO on nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments using age synchronized young adult C. elegans population exposed to GO and rGO for 24h. We used whole genome microarrays to screen for global changed in C. elegans transcription profiles and with subsequent quantitative analysis conducted on selected genes. Young adults of C.elegans were selected for RNA extraction and hybridization on Affymetrix microarrays.