Project description:Gene expression profiles at different development stages and different growth medium of Lentinula edodes (L54) are comparied. Keywords: time-course
Project description:Gene expression profiles at different development stages and different growth medium of Lentinula edodes (L54) are comparied. Keywords: time-course
Project description:Gene expression profiles before and after spore formation of Lentinula edodes (L54)grown at sawdust. Keywords: time-course SAGE were used to generate tags from RNA of fruit bodies of L. edodes. RNA were extracted from the fruit bodies before and after spore observed. Gene expression profiles of both stages were compared to screen out genes may relate to spore formation.
Project description:Gene expression profiles before and after spore formation of Lentinula edodes (L54) grown at sawdust. SAGE were used to generate tags from RNA of fruit bodies of L. edodes. RNA were extracted from the fruit bodies before and after spore observed. Gene expression profiles of both stages were compared to screen out genes may relate to spore formation. To facilitate comparison of LongSAGE and SAGE data in GEO, LongSAGE tags (15bp) were trimmed to become 10bp and uploaded in previous submission (GSM87320, GSM87321). The current submission are actual LongSAGE experimental data without any extrapolation from SAGE data.
Project description:Some mushroom proteins and peptides have reported biological activities increasing interest due to their potential as nutraceuticals. It is necessary to research optimizing the substrates for biomass and protein production to improve the biotechnological approaches. This study evaluated the effect on biomass production and total intra-protein in Lentinula edodes grown by liquid-state fermentation (LSF) for eight culture mediums. The higher total protein content was found on bienestarina media (BIE), glucose, peptone, and yeast extract media (GPY). The protein profile using a shotgun proteomics approach by LC-MSMS showed the media's marked influence on protein production. Most proteins identified using the BIE medium did not appear when the mushroom grew in the GPY. Some of the detected proteins included lectins and enzymes, are used in industrial applications. These results would indicate that BIE promotes in L. edodes the production of proteins with potential for its application to the biotechnology industry
