Project description:We present a label free proteome dataset of the vascular sap proteome of three commercially important Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus urophylla). Protein extraction from the vascular system was carried out using a pressure bomb, in solution digested and peptides were analyzed using a Q-Exactive instrument. Protein identification was carried out using stringent database searches and only in silico predicted extracellular proteins were considered as part of the sap proteome. The results here described can be used as a reference for the proteome sap analysis of Eucalyptus plants grown under different conditions.
Project description:The secondary xylem of trees serves as the fundamental biological foundation for wood production, and unraveling its regulatory mechanisms has consistently remained a central scientific challenge in basic forest research. In this project, iTraq quantitative proteomics technology was used to analyze proteins related to secondary xylem differentiation in the immature xylem of E. urophylla × E. grandis (E. urograndis) at different ages in response to changes in the age of the trees.