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Eucalyptus urophylla x Eucalyptus grandis

ABSTRACT: RNA-seq analysis of hybrid Eucalyptus with contrasting wood basic density

PROVIDER: PRJDB5299 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR081574_1.fastq.gz	Fastqsanger.gz
	DRR081574_2.fastq.gz	Fastqsanger.gz
	DRR081575_1.fastq.gz	Fastqsanger.gz
	DRR081575_2.fastq.gz	Fastqsanger.gz

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Eucalyptus urophylla x Eucalyptus grandis

Project description:Transcriptional profiles of hybrid Eucalyptus genotypes with contrasting lignin content

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Vascular sap proteome profiling of Eucalyptus grandis, Eucalyptus urophylla and Eucalyptus camaldulensis

Project description:We present a label free proteome dataset of the vascular sap proteome of three commercially important Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus urophylla). Protein extraction from the vascular system was carried out using a pressure bomb, in solution digested and peptides were analyzed using a Q-Exactive instrument. Protein identification was carried out using stringent database searches and only in silico predicted extracellular proteins were considered as part of the sap proteome. The results here described can be used as a reference for the proteome sap analysis of Eucalyptus plants grown under different conditions.

2025-05-30 | PXD037915 | Pride

Proteomic analysis in Eucalyptus urophylla × Eucalyptus grandis

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Eucalyptus grandis x Eucalyptus urophylla

Project description:Eucalyptus grandis x Eucalyptus urophylla

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Project description:The intense human activities can cause irreversible environmental problems. Eucalyptus is a forest species widely used in planted forests, with a great capacity to assist in the mitigation of CO2 emissions and accumulation due to its C3 metabolism and high retention of carbon molecules in its biomass. The objective of this study was to investigate the differences in the sap proteome of two Eucalyptus species grown in an atmosphere enriched with CO2. For this purpose, young Eucalyptus grandis and Eucalyptus urophylla plants were grown in growth chambers 20 days under controlled atmospheric CO2 rates. The vascular proteome revealed 146 extracellular proteins, and their relative abundance was associated with the enriched atmosphere treatments. The analysis of protein function and abundance revealed that E. grandis proteins are mainly involved in organic substance metabolism and proteolysis, while less abundant proteins are related to cellular defense responses. Similar results were obtained for E. urophylla, with the most abundant proteins performing metabolic functions, while the least abundant protein was related to oxidative stress. These results may contribute to a better understanding of the mechanisms involved in the response of eucalyptus species to increased CO2 and provide useful information for the management and cultivation of these species in high levels of carbon dioxide environments.

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Eucalyptus urophylla x Eucalyptus grandis

Project description:Eucalyptus urophylla x Eucalyptus grandis Assembly - Alternate haplotype

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Project description:Fast-growing Eucalyptus grandis trees are one of the most efficient producers of wood in South Africa. It is essential to maximize the effectiveness of these plantations by increasing their productivity, the quality and value of their products. We used microarray-based DNA-amplified fragment length polymorphism (AFLP) analysis in combination with expression profiling to develop fingerprints and profile gene expression of wood-forming tissue of seven individual E. grandis trees. A 1532-probe cDNA microarray was constructed by arraying 768 cDNA-AFLP fragments and 810 cDNA library clones from seven individual E. grandis trees onto silanised slides. The results revealed that 32% of the spotted fragments showed distinct expression patterns (with a fold change of at least 1.4 or -1.4 and a p value of 0.01) and could be grouped into clusters representing co-expressed genes. Evaluation of the binary distribution of cDNA-AFLP fragments on the array showed that the individual genotypes could be discriminated. A simple, yet general method was developed for genotyping and expression profiling of wood-forming tissue of E. grandis trees differing in their splitting characteristics and in their lignin contents. Evaluation of gene expression profiles and the binary distribution of cDNA-AFLP fragments on the chip suggest that the prototype chip developed could be useful for transcript profiling and for the identification of Eucalyptus trees with preferred wood quality traits in commercial breeding programmes.

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