Project description:CHO cells have primarily been studied in bulk, assuming that these bulk samples are identical because of genetic clonality across the sample. In this study, we performed single-cell RNA sequencing on two clonal CHO-K1 cell populations with different stability phenotypes over a 90 day culture period.
Project description:RNA-sequencing was performed on H1299, HEK293T and CHO-K1 transfected with plasmids expressing EGFP and mKate in an open loop circuit (OL), w/wo compound supplementation, or with iFFL circuit.mKate has target sites for miRNAs highly expressed in the different cell lines (miRNA31 for H1299, miRNA221 for HEK293T and miRNA21 for CHO-K1)