Project description:Objectives: determination of transcription start sites in Vibrio harveyi genome and discovery of new transcripts Methods: we performed differential seqencing of total RNA isolated from o.n. control Vibrio harveyi cultures. Sample treatment with Terminator EXonuclease (TEX) allowed differenciation of primary and secondary transcripts, helping in the definition of transcription start sites (TSS) Results: by data-mining RNA-seq data and performing some Northern Blot experiments we were able to detect new putative small-RNAs, along with these results, a more deep analisys of our RNA-seq data will give futher insight into genetic organization of Vibrio harveyi genome to help in its investigation
Project description:In this research, we used RNA-sequencing technology to detect genome-wide differentially expressed genes in spleen and gill of Vibrio harveyi -infected Takifugu rubripes.This high-throughput sequencing could help us to understand new mechanisms of action of V. harveyi induced aquaculture fish disease.
Project description:Vibrio harveyi is a major bacterial pathogen that can cause fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis). To comprehend the molecular mechanisms of C. semilaevis host response against V. harveyi infection, we performed transcriptome (RNA-seq) analysis of C. semilaevis from resistant family and susceptible family.
Project description:Vibrio harveyi, a significant pathogen in aquaculture, can lead to severe gut damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the gut damage caused by Vibrio harveyi in Hybrid Groupers and the Alleviating Effect of oligochitosan Feeding.
Project description:Vibrio harveyi, a significant pathogen in aquaculture, can lead to severe liver damage and metabolic disorders in fish. Verification via Transcriptome Sequencing of the Hepatic Damage Caused by Vibrio harveyi in Hybrid Groupers and the Alleviating Effect of Chitosan Oligosaccharide Feeding.
Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe organ damage and metabolic disorders in fish. The organ damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
Project description:Vibrio harveyi is an important pathogen in aquaculture, which can cause severe intestinal damage and metabolic disorders in fish. The intestinal damage of hybrid grouper caused by Vibrio harveyi was verified through transcriptional analysis.
