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Hordeum vulgare

ABSTRACT: RNA-seq analysis in callus of barley

PROVIDER: PRJDB7393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR149321.fastq.gz	Fastqsanger.gz
	DRR149322.fastq.gz	Fastqsanger.gz
	DRR149323.fastq.gz	Fastqsanger.gz
	DRR149324.fastq.gz	Fastqsanger.gz
	DRR149325.fastq.gz	Fastqsanger.gz

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Hordeum vulgare Transcriptome or Gene expression

Project description:We hypothesized that the genome segments of cultivated barley should show certain similarity with its ancestral wild barley. Instead of whole genome sequences, we employed RNA-Seq to investigated the genomic origin of modern cultivated barley using some representative wild barley genotypes from the Near East and Tibet, and representative world-wide selections of cultivated barley.

2014-10-06 | E-MTAB-4221 | biostudies-arrayexpress

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Hordeum vulgare strain:landrace Transcriptome or Gene expression

Project description:In the present study, we investigated the transcriptome features during hulless barley grain development. Using Illumina paired-end RNA-Sequencing, we generated two data sets of the developing grain transcriptomes from two hulless barley landraces.

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Genome-Wide Analysis of the MADS-box Gene Family Involved in Salt and Waterlogging Tolerance in Barley (Hordeum Vulgare. L)

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Hordeum vulgare

Project description:Hulless barley DAP-seq rawdata

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Transcriptomic changes in the crown (stem base) of barley (Hordeum vulgare L. cv. Golden Promise) during crown-root initiation and development

Project description:Purpose: The goal of this study was to investigate the mechanisms involved in the initiation and development of crown-roots (CRs) in barley and to estimate the role of cytokinins (CKs) in this process. Method: stranded libraries were obtained from RNA extracted from the stem base of 1 day-after-germination (DAG) and 10DAG-seedlings of wild-type (WT) and AtCKX-overexpressing barley lines (OE-CKX). OE-CKX lines have a reduced content of endogenous CKs and are characterized by a higher number of CRs. Libraries were deep sequenced on Illumina HighSeq platform. Each sample was investigated in three independent biological replicates. Results: Using a data analysis workflow optimized for barley, we identified more than 4000 transcripts differentially expressed in the stem base of 1DAG and 10DAG-seedlings. Expression as determined by RNA-seq was validated by real-time PCR. Our data were compared to the transcriptomic profiling obtained from rice and we were able to identify genes potentially involved in the initiation/development of CRs in barley. Also the use of the transgenic line with altered endogenous CK content allowed us to conclude about the role of CKs in the process. Conclusions: Our study represents the first analysis aiming to understand the initiation and development of CRs in barley.

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