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Polypedilum vanderplanki

ABSTRACT: Transcriptomic analysis of anhydrobiosis for cultured cell line, Pv11, from Polypedilum vanderplanki

PROVIDER: PRJDB7516 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR153208.fastq.gz	Fastqsanger.gz
	DRR153209.fastq.gz	Fastqsanger.gz
	DRR153210.fastq.gz	Fastqsanger.gz
	DRR153211.fastq.gz	Fastqsanger.gz
	DRR153212.fastq.gz	Fastqsanger.gz

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Project description:Pv11 is an insect cell line established from Polypedilum vanderplanki with ability to survive complete desiccation if they were treated with trehalose. Previous studies showed contribution of HSF1 gene in this adaptation. To determine HSF1 regulatory network in Pv11 cells we prepared RNAseq libraries for wild type Pv11 (WT), HSF1 knoked out (Hsf1-/-), HSf1 resqued (Hsf1-/-; Hsf1-HaloTag/BlaR), and negative control for HSF1 resqued cells (Hsf1-/- HaloTag/BlaR) under normal conditions and under incubation with trehalose for 48 h. Comparison of mRNA profiles showed that HSF1 induced anhydrobiosis-related genes, especially LEA and TRX, but suppressed cellular process-related genes, for example transcription- and translation-related genes.

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