Project description:To identify the mechanism of Microbial Influenced Corrosion (MIC) and the bacterial response toward corrosion, we conducted whole genome microarray expression profile. At log phase, the cell of Clostridium carboxidivorans using iron granule as an electron donor (corroding iron) was collected as a sample, and that of using syngas as an electron donor was collected as a control.
Project description:Microbial induced mineral precipitations caused by nitrate treatment for souring control during microbial enhanced oil recovery (MEOR)
Project description:Samples collect to investigate the gene activity from microbial populations in marine steel corrosion, and to compare with gene activity in water and bed sediment samples from the surrounding area. The study was undertaken to (1) investigate mechanisms of microbially influenced corrosion (MIC) of marine steel, and (2) compare microbial population gene activity between corrosion and the surrounding environment. Purified DNA (1µg) was labelled with Cy3, purified and hybridised at 42°C for 16h with the GeoChipTM 5.0 on a MAUI hybridisation station (BioMicro, USA).
Project description:16s RNA gene sequencing data from seawater, bed sediment and steel corrosion samples from Shoreham Harbour, UK, collected to allow bacterial species comparisons between microbially influenced corrosion, the surrounding seawater, and the sea bed sediment at the seafloor and 50cm depth below seafloor.
Project description:Systemic sclerosis (SSc) is characterized by intractable multiorgan fibrosis caused by vascular and immune dysfunction. Currently, effective therapeutic options for patients with SSc are limited. Nitrate, an abundant nutrient in the diet, has been demonstrated to be preventative and therapeutic for several diseases. To determine whether nitrate can slow or reverse SSc progression, topical application of nitrate delivered by dissolving microneedles was used to treat a bleomycin (BLM)-induced dermal fibrosis mouse model. In this study, nitrate considerably attenuated dermal thickness, stiffness, and collagen deposition.To examine the nitrate regulation of gene expression at the genome-wide level, bulk RNA sequencing of skin was performed. Bulk RNA sequencing of skin revealed that Cd4 was a key hub gene in SSc nitrate therapy. Additionally, BLM-induced cytokines and chemokines were inhibited by nitrate, and CD4+ T cells infiltration markedly declined. Il4, Il6, Il13, and Tgfb expression in CD4+ T cells isolated from skin biopsies also significantly decreased. Mechanistically, Il1rl1, a type2 immune response inducer, was markedly repressed in isolated CD4+ T cells and dermal tissues after nitrate treatment. Remarkably, compared with wild type mice, mice lacking Il1rl1 showed impaired transcriptional profiles after intradermal BLM injection. Adoptive transfer of ST2+CD4+ T cells promoted bleomycin-induced Rag2-/- mice dermal fibrosis. Collectively, these findings demonstrate that nitrate targeting ST2+CD4+ T cells is an effective therapeutic option for SSc.
Project description:Microbial extracellular electron uptake (EEU) is central to bioelectrochemical processes and biocorrosion, yet its molecular mechanisms remain incompletely understood. Here, we investigate how excess Fe2+ modulates EEU in Desulfovibrio ferrophilus IS5, a strain that causes severe anaerobic iron corrosion via outer-membrane cytochromes (OMCs)-mediated electron uptake. We show that IS5 grown with elevated Fe2+ exhibits substantially enhanced EEU. This enhancement arises through two complementary mechanisms: (i) increased abundance of functional OMCs via upregulation of a cytochrome assembly protein, and (ii) an additional electron transfer route mediated by FeS nanoparticles precipitated on the IS5 outer membrane. Remarkably, IS5 with low OMCs expression but biosynthesized FeS can rapidly shift to EEU before OMCs induction. These findings suggest that during iron corrosion, when IS5 cells are embedded within thick corrosion crusts and biofilms and face both high Fe2+ concentrations and organic limitation, they exploit OMCs and FeS nanoparticles in parallel to sustain high-rate EEU from iron. This study advances the mechanistic understanding of EEU-driven iron corrosion and highlights a potential avenue for manipulating bioelectrochemical systems.
