Aspergillus
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REPOSITORIES: ENA
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Project description:Transcriptome analysis of Aspergillus section Fumigati
| PRJDB7496 | ENA
Project description:Decoding Azole Resistance Mechanisms and Pathogenicity in Aspergillus Section Fumigati
| PRJNA1234068 | ENA
Project description:Biological Relevance and Intent: Cryptic species within the Aspergillus section Fumigati, such as the Aspergillus viridinutans species complex, are increasingly recognized as important opportunistic pathogens in both human and veterinary medicine. These species often exhibit an intrinsic reduction in susceptibility to standard azole antifungals. The intent of this experiment was to determine how the tolerant veterinary isolate Aspergillus felis CCF5624 responds to acute azole stress (tolerance is transient state, not genetically determined like mutations). This study aimed to map the active cellular survival pathways and identify regulatory networks that could be targeted to bypass this protective tolerance machinery. Experimental Workflow Overview: Conidia suspensions of Aspergillus felis CCF5624 were inoculated onto pure RPMI medium covered with sterile cellophane and cultivated for 40 hours at 37 °C to establish vegetative colonies. To capture the immediate transcriptomic shifts associated with drug tolerance, the grown biomass was transferred directly to new RPMI plates supplemented with either a control solvent (DMSO) [abbreviated as \"A\"] or one of three clinical azole compounds (itraconazole, voriconazole, or posaconazole) [abbreviated as B, C,D, respectively] at concentrations corresponding to EUCAST resistance breakpoints. Following an acute 4-hour exposure, fungal biomass was harvested and flash-frozen in liquid nitrogen. Total RNA was isolated using a combined TRI reagent and spin-column methodology. High-throughput RNA sequencing (RNA-seq) was performed by Novogene Limited UK. Poly-A enriched mRNA libraries were prepared and sequenced on the Illumina NovaSeq X Plus platform using a 150 bp paired-end (PE150) strategy, yielding a minimum sequencing depth of 3 Gb (approximately 10 million paired-end reads) per individual sample. Reference gemone Aspergillus pseudoviridinutans IFM_55266 https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/018/340/605/GCF_018340605.1_Asppvi_assembly01/GCF_018340605.1_Asppvi_assembly01_genomic.fna.gz https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/018/340/605/GCF_018340605.1_Asppvi_assembly01/GCF_018340605.1_Asppvi_assembly01_genomic.gtf.gz
2026-07-15 | E-MTAB-17342 | biostudies-arrayexpress
Project description:Aspergillus flavus and A. parasiticus are two of the most important aflatoxin-producing species that contaminate agricultural commodities worldwide. Both species are heterothallic and undergo sexual reproduction in laboratory crosses. Here, we examine the possibility of interspecific matings between A. flavus and A. parasiticus. These species can be distinguished morphologically and genetically, as well as by their mycotoxin profiles. Aspergillus flavus produces both B aflatoxins and cyclopiazonic acid (CPA), B aflatoxins or CPA alone, or neither mycotoxin; Aspergillus parasiticus produces B and G aflatoxins or the aflatoxin precursor O-methylsterigmatocystin, but not CPA. Only four out of forty-five attempted interspecific crosses between compatible mating types of A. flavus and A. parasiticus were fertile and produced viable ascospores. Single ascospore strains from each cross were isolated and were shown to be recombinant hybrids using multilocus genotyping and array comparative genome hybridization. Conidia of parents and their hybrid progeny were haploid and predominantly monokaryons and dikaryons based on flow cytometry. Multilocus phylogenetic inference showed that experimental hybrid progeny were grouped with naturally occurring A. flavus L strain and A. parasiticus. Higher total aflatoxin concentrations in some F1 progeny strains compared to midpoint parent aflatoxin levels indicate synergism in aflatoxin production; moreover, three progeny strains synthesized G aflatoxins that were not produced by the parents, and there was evidence of putative allopolyploidization in one strain. These results suggest that hybridization is an important diversifying force resulting in the genesis of novel toxin profiles in these agriculturally important species.
2015-10-01 | GSE61981 | GEO
Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.
2005-05-15 | E-MEXP-333 | biostudies-arrayexpress
Project description:Using transcriptomics, the strain-specific metabolism was mapped for two whole-genome sequenced strains of Aspergillus niger Keywords: Strain comparison
2011-03-07 | GSE10983 | GEO
Project description:Mycovirus identification from Aspergillus section Versicolores
| PRJDB12199 | ENA
Project description:The filamentous fungus Aspergillus oryzae is an important microbial cell factory for industrial production of useful enzymes, such as α-amylase. In order to optimize the industrial enzyme production process, there is a need to understand fundamental processes underlying protein production, here under how protein production links to metabolism through global regulatory structures. In this study, two α-amylase-producing strains of A. oryzae, a wild type strain and a transformant strain containing additional copies of the α-amylase gene, were characterized at a systematic level. Based on integrated analysis of ome-data together with genome-scale metabolic network and flux calculation, we identified key genes, key enzymes, key proteins, and key metabolites involved in the processes of protein synthesis and secretion, nucleotide metabolism, and amino acid metabolism that can be the potential targets for improving industrial protein production. Keywords: Two Aspergillus oryzae strains and two different carbon sources
2012-02-12 | GSE22566 | GEO