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Bombyx mori strain:BmN cell

ABSTRACT: Zucchini dependent and independent pre-piRNA generation and their sequence features in silkworms

PROVIDER: PRJDB8413 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR181856_1.fastq.gz	Fastqsanger.gz
	DRR181856_2.fastq.gz	Fastqsanger.gz
	DRR181857.fastq.gz	Fastqsanger.gz
	DRR181858.fastq.gz	Fastqsanger.gz
	DRR181859.fastq.gz	Fastqsanger.gz

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Gene expression profiling of the fat body of Bombyx mori exposed to phoxim insecticide

Project description:Transcriptional characteristics of genes in the fat body of domestic silkworms after 24 h exposure to phoxim through whole-genome oligonucleotide microarray.

2016-01-01 | GSE60877 | GEO

Gene expression profiling of the fat body of Bombyx mori exposed to phoxim insecticide

Project description:Transcriptional characteristics of genes in the fat body of domestic silkworms after 24 h exposure to phoxim through whole-genome oligonucleotide microarray. Transcriptional profiling of fat body in the domestic silkworms comparing control untreated fat body with phoxim-treated fat body Transcription profiling experiments, phoxim-treated fat body (samples) were analyzed. Dual-channel experiments, with test samples labeled by Cy5 and control samples labeled by Cy3. Three Biological replicate. No dye-swaps.

2016-07-03 | E-GEOD-60877 | biostudies-arrayexpress

Bombyx mori

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piRNA-guided slicing specifies transcripts for Zucchini dependent, phased piRNA biogenesis

Project description:PIWI-clade Argonaute proteins repress transposable elements in animal gonads. Their sequence specificity is conferred via bound ~23-30nt long piRNAs, which are processed from single stranded precursor RNAs. How transcripts are specified as precursors and processed into stereotypical piRNA populations are central unresolved questions. Here we show that piRNA-guided RNA cleavage in Drosophila results not only in generation of a ping-pong partner piRNA but further triggers efficient 3′ directed and phased primary piRNA biogenesis. Phasing is a feature of primary piRNAs in somatic and germline cells and a consequence of consecutive endo-nucleolytic cleavage events catalyzed by Zucchini. Formation of 3′ and 5′ ends of flanking piRNAs is therefore tightly coupled. Zucchini also participates in 3′ end formation of secondary piRNAs but its function can be bypassed by additional downstream piRNA-guided cleavages and subsequent precursor trimming. Hallmarks of Zucchini-dependent phased piRNA biogenesis are also evident in mouse testes, pointing to an evolutionarily conserved mechanism of piRNA biogenesis. This study aims at understanding how piRNA biogenesis is intiated in the Drosophila germline and understanding the role of the nuclease Zucchini/MitoPLD in piRNA biogenesis in Drosophila/Mouse by analysing small RNA sequencing data of various genotypes and sensor constructs.

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