Project description:Comparative Genomic Hybridization. Analysis of genomic content of closely related Bacillus species. Refer to individual records for strain information. Refer to platform and individual sample records for experimental protocols. Keywords: other
Project description:The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. anthracis strains.
Project description:Enterocin AS-48 is produced by Enterococcus faecalis S48 to compete with other bacteria in their environment. Due to its activity against various Gram positive and some Gram negative bacteria it has clear potential for use as a food preservative. Here, we studied the effect of enterocin AS-48 challenges on vegetative cells of Bacillus cereus ATCC 14579 by use of transcriptome analysis.
Project description:Bacterial response to nitric oxide (NO) is of major importance for bacterial survival, as NO stress is a main actor of the eukaryote immune response. Several pathogenic bacteria have developed gene regulation systems involved in detoxification and in repairing the damages caused by NO. However, bacterial mechanisms of NO resistance are poorly described for Gram positive bacteria and especially Bacillus cereus. This food born and opportunistic pathogen does not have the common weapon against NO described in other pathogenic or non-pathogenic bacteria, such as the transcriptional regulators norR and nsrR, nor NO reductases. Using a transcriptomic approach, we investigated the mechanisms used by B. cereus to fight against NO stress. A cluster of 6 genes was identified to be particularly overexpressed in the early response to NO stress. This cluster contains an iron-sulphur cluster repair enzyme, a nitrite reductase and three enzymes involved in siroheme biosynthesis. We show a co-expression and a close genetic localization implying a functional link between those genes. This cluster may play a pivotal role in the defence mechanisms used by B. cereus to fight against NO stress during infection.
Project description:Enterocin AS-48 is produced by Enterococcus faecalis S48 to compete with other bacteria in their environment. Due to its activity against various Gram positive and some Gram negative bacteria it has clear potential for use as a food preservative. Here, we studied the effect of enterocin AS-48 challenges on vegetative cells of Bacillus cereus ATCC 14579 by use of transcriptome analysis. Control: Wild type against Target: Enterocin AS-48 challenge A supplementary file containing processed data of all samples combined is linked below. Mn column is the lowess normalized LN (Target/Control).
