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Physcomitrium patens

ABSTRACT: Identification of the R-loop regions in the moss Physcomittella patens

PROVIDER: PRJDB9556 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR218227_1.fastq.gz	Fastqsanger.gz
	DRR218227_2.fastq.gz	Fastqsanger.gz
	DRR218228_1.fastq.gz	Fastqsanger.gz
	DRR218228_2.fastq.gz	Fastqsanger.gz
	DRR218229_1.fastq.gz	Fastqsanger.gz

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Small RNAs from the moss Physcomitrella patens

Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing

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Project description:Here, we provide an isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic dataset of the moss Physcomitrium patens smg1 knockout line. The kinase SMG1 is one of the key components of the NMD system in many organisms, including plants. Several lines with a deleted SMG1 in a land Physcomitrium patens subsp. patens (“Gransden 2004”, Frieburg) were produced by James P. B. Lloyd [doi.org/10.1111/tpj.12329]. One of these lines, SMG1 KO mutant line 2, was used for this study. Protonemata of WT and mutant lines were grown in200 ml liquid BCD medium supplemented with 5 mM ammonium tartrate (BCDAT) during a 16-h photoperiod at 250C for 8 days [https://doi.org/10.1101/pdb.prot5136]. All experiments were performed in three biological replicates.

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The Physcomitrium patens egg cell changes the epigenetic program and utilizes homologs of Arabidopsis male genes, including BONOBOs, for cell specification

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