Mus musculus
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR228577_1.fastq.gz | Fastqsanger.gz | |||
| DRR228577_2.fastq.gz | Fastqsanger.gz | |||
| DRR228578_1.fastq.gz | Fastqsanger.gz | |||
| DRR228578_2.fastq.gz | Fastqsanger.gz | |||
| DRR228579_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 38 |
Similar Datasets
Project description:Round spermatid injection (ROSI) gives rise to a lower birth rate than intracytoplasmic sperm injection (ICSI), which uses mature spermatozoa and has limited clinical application. Inefficient development of ROSI-embryos has been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins the low birth rate of ROSI remains to be determined. Here we show that a repressive histone mark H3K27me3 persists from doner round spermatids into zygotes in ROSI and that paternally derived H3K27me3 is associated with less accessible chromatin and impaired zygotic genome activation (ZGA) in ROSI-derived zygotes. These H3K27me3 marked loci in the paternal germline undergo histone turnover in spermiogenesis, resulting in the reduced paternal H3K27me3 in normal spermatozoa. Thus, the lack of histone turnover during spermiogenesis led to the dysregulation of chromatin accessibility and ZGA in ROSI-embryos. Our results unveil a molecular logic by which chromatin states in round spermatids impinge on chromatin states and ZGA in ROSI-embryos, highlighting the importance of chromatin remodeling in spermiogenesis in successful reproduction.
2022-08-23 | GSE196858 | GEO
Project description:Round spermatid injection (ROSI) gives rise to a lower birth rate than intracytoplasmic sperm injection (ICSI), which uses mature spermatozoa and has limited clinical application. Inefficient development of ROSI-embryos has been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins the low birth rate of ROSI remains to be determined. Here we show that a repressive histone mark H3K27me3 persists from doner round spermatids into zygotes in ROSI and that paternally derived H3K27me3 is associated with less accessible chromatin and impaired zygotic genome activation (ZGA) in ROSI-derived zygotes. These H3K27me3 marked loci in the paternal germline undergo histone turnover in spermiogenesis, resulting in the reduced paternal H3K27me3 in normal spermatozoa. Thus, the lack of histone turnover during spermiogenesis led to the dysregulation of chromatin accessibility and ZGA in ROSI-embryos. Our results unveil a molecular logic by which chromatin states in round spermatids impinge on chromatin states and ZGA in ROSI-embryos, highlighting the importance of chromatin remodeling in spermiogenesis in successful reproduction.
2022-08-23 | GSE196857 | GEO
Project description:Round spermatid injection (ROSI) results in a lower birth rate than intracytoplasmic sperm injection, which has hampered its clinical application. Inefficient development of ROSI embryos has been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins the low birth rate in ROSI remains to be determined. Here, we show that a repressive histone mark H3K27me3 persists from mouse round spermatids into zygotes in ROSI and that round spermatid-derived H3K27me3 is associated with less accessible chromatin and impaired gene expression in ROSI embryos. These loci are initially marked by H3K27me3 but undergo histone modification remodelling in spermiogenesis, resulting in reduced H3K27me3 in normal spermatozoa. Therefore, the absence of the epigenetic remodelling, presumably mediated by histone turnover during spermiogenesis, leads to dysregulation of chromatin accessibility and transcription in ROSI embryos. Thus, our results unveil a molecular logic, in which chromatin states in round spermatids impinge on chromatin accessibility and transcription in ROSI embryos, highlighting the importance of epigenetic remodelling during spermiogenesis in successful reproduction.
2022-08-22 | GSE196859 | GEO
Project description:Zygotic genome activation (ZGA), which is according to the midblastula transition in zebrafish, is an important event during the maternal-zygotic transition in animals. Our preliminary study and other groupâs works indicate that epigenetic regulations play an essential role in ZGA. Morpholino was employed to knockdown PRMT6. We used microarrays to analyze the global gene expression in prmt6 morphants. prmt6 MO (0.3mM) was injected into the one-two cell zebrafish, prmt6 cMO (0.3mM) injection as a control. At 6 hpf, embryos were classified into three subtypes (normal, mild and severe) and prepared for global gene expression analysis with Affymetrix Zebrafish Genome Arrays. The severe subtype and the control were repeated three times.
2015-11-25 | E-GEOD-63735 | biostudies-arrayexpress
Project description:Paternally inherited H3K27me3 impacts zygotic genome activation in round spermatid injection [RNA-seq]
| PRJNA807596 | ENA
Project description:Paternally inherited H3K27me3 impacts zygotic genome activation in round spermatid injection [ATAC-seq]
| PRJNA807599 | ENA
Project description:Early zebrafish embryo development proceeds first from a maternally transcribed and stored mRNAs, and zygotic gene activation (ZGA) is initiated at the mid-blastula transition (MBT; 1000-cell stage), 3.3 h post-fertilization. Very little is known on how the zygotic genome is programmed for transcriptional activation at the MBT. To start addressing this issue, we have mapped by ChIP-chip genome-wide promoter histone methylation (H3K4me3, H3K9me3, H3K27me3, H3K36me3) and RNA Pol II profiles before ZGA (256-cell stage; 2.5 hpf), during ZGA (MBT; 3.5 hpf)) and after ZGA (Post-MBT; 5.3 hpf) . We used a custom 2.1M probe HD promoter array (Nimblegen) for ChIP and input DNA hybridization. Peak detection was done using MA2C with P=10e-4 as cutoff. ChIP-chip experiments were performed from chromatin prepared by sonication after formaldehyde cross-linking, from embryos are the indicated developmental stages and ChIP DNA was hybridized onto the aforementioned Nimbegen promoter arrays.
2011-12-02 | E-GEOD-27314 | biostudies-arrayexpress
Project description:Zygotic genome activation (ZGA) represents the inaugural post-fertilization transcription event. In mouse, RNA polymerase II (Pol II) initiates embryonic transcription through a step-wise transition, but the ZGA initiation process remains unclear in non-rodents. Here, Pol II profiling in bovine and porcine embryos, combined with multi-omics data across four mammalian species, revealed strong intergenic Pol II clusters, termed super Pol II domains (SPDs), that boost minor ZGA gene expression via chromatin interactions in bovine. Intriguingly, a similar strategy was found in human embryos, which also exhibit delayed ZGA followed by rapid up-regulation of minor ZGA genes. By contrast, these genes are precociously activated in porcine and mouse gametes before fertilization. Moreover, disruption of SPD structure, which decreases two minor ZGA genesKLF17orDUXAexpression, and knockdown them in bovine, leads to aberrant gene activation and impeded embryogenesis. Our findings highlight SPD enhancement as a species-specific evolutionary adaptation for initiating transcription in bovine and human embryos, thus illustrating previously underappreciated diversity in mammalian reproductive developmental strategies.
2025-05-01 | GSE264469 | GEO
Project description:Zygotic genome activation (ZGA) represents the inaugural post-fertilization transcription event. In mouse, RNA polymerase II (Pol II) initiates embryonic transcription through a step-wise transition, but the ZGA initiation process remains unclear in non-rodents. Here, Pol II profiling in bovine and porcine embryos, combined with multi-omics data across four mammalian species, revealed strong intergenic Pol II clusters, termed super Pol II domains (SPDs), that boost minor ZGA gene expression via chromatin interactions in bovine. Intriguingly, a similar strategy was found in human embryos, which also exhibit delayed ZGA followed by rapid up-regulation of minor ZGA genes. By contrast, these genes are precociously activated in porcine and mouse gametes before fertilization. Moreover, disruption of SPD structure, which decreases two minor ZGA genesKLF17orDUXAexpression, and knockdown them in bovine, leads to aberrant gene activation and impeded embryogenesis. Our findings highlight SPD enhancement as a species-specific evolutionary adaptation for initiating transcription in bovine and human embryos, thus illustrating previously underappreciated diversity in mammalian reproductive developmental strategies.
2025-05-01 | GSE264460 | GEO
Project description:Zygotic genome activation (ZGA) represents the inaugural post-fertilization transcription event. In mouse, RNA polymerase II (Pol II) initiates embryonic transcription through a step-wise transition, but the ZGA initiation process remains unclear in non-rodents. Here, Pol II profiling in bovine and porcine embryos, combined with multi-omics data across four mammalian species, revealed strong intergenic Pol II clusters, termed super Pol II domains (SPDs), that boost minor ZGA gene expression via chromatin interactions in bovine. Intriguingly, a similar strategy was found in human embryos, which also exhibit delayed ZGA followed by rapid up-regulation of minor ZGA genes. By contrast, these genes are precociously activated in porcine and mouse gametes before fertilization. Moreover, disruption of SPD structure, which decreases two minor ZGA genesKLF17orDUXAexpression, and knockdown them in bovine, leads to aberrant gene activation and impeded embryogenesis. Our findings highlight SPD enhancement as a species-specific evolutionary adaptation for initiating transcription in bovine and human embryos, thus illustrating previously underappreciated diversity in mammalian reproductive developmental strategies.
2025-05-01 | GSE264467 | GEO