Arctica islandica
Ontology highlight
ABSTRACT:
PROVIDER: PRJEA89475 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Most crop species cannot survive flooding events for a long period of time. However, within the Cardamineae tribe of the Brassicaceae family, there exist several wild species with high flooding tolerance. Here, Rorippa islandica was identified as a genetically accessible diploid species with high submergence tolerance. Another diploid species from the same genus, R. stylosa, showed similar submergence sensitivity compared with Arabidopsis. Both species exhibited a strong and partially overlapping transcriptomic response to submergence within the first 48 hours of stress, including carbon-starvation and ethylene-responsive marker genes. We demonstrate that R. islandica can be transformed via floral dip, but with rather low frequency. The successful CRISPR-Cas9-mediated knockout of RiBCA3 confirms the suitability of this species for genetic transformation. However, although it was hypothesized that RiBCA3 might have an important function in carbon fixation under water, no differences in submergence survival or underwater photosynthesis were observed between wildtype and bca3 knockout lines. The molecular mechanisms of submergence tolerance of Rorippa islandica are therefore not understood yet. The data indicate that illumination and therefore photosynthesis-associated processes must be the basis for superior submergence survival. This work suggests that Rorippa islandica is a promising dicot model to further investigate the underlying tolerance mechanisms.
2025-12-31 | GSE295090 | GEO
Project description:Arctica islandica is known as the longest-lived non-colonial metazoan species on earth and is therefore increasingly being investigated as a new model in aging research. As the mitochondrial genome is associated with the process of aging in many species and bivalves are known to possess a peculiar mechanism of mitochondrial genome inheritance including doubly uniparental inheritance (DUI), we aimed to assess the genomic variability of the A. islandica mitochondrial DNA (mtDNA). We sequenced the complete mitochondrial genomes of A. islandica specimens from three different sites in the Western Palaearctic (Iceland, North Sea, Baltic Sea). We found the A. islandica mtDNA to fall within the normal size range (18 kb) and exhibit similar coding capacity as other animal mtDNAs. The concatenated protein sequences of all currently known Veneroidea mtDNAs were used to robustly place A. islandica in a phylogenetic framework. Analysis of the observed single nucleotide polymorphism (SNP) patterns on further specimen revealed two prevailing haplotypes. Populations in the Baltic and the North Sea are very homogenous, whereas the Icelandic population, from which exceptionally old individuals have been collected, is the most diverse one. Homogeneity in Baltic and North Sea populations point to either stronger environmental constraints or more recent colonization of the habitat. Our analysis lays the foundation for further studies on A. islandica population structures, age research with this organism, and for phylogenetic studies. Accessions for the mitochondrial genome sequences: KC197241 Iceland; KF363951 Baltic Sea; KF363952 North Sea; KF465708 to KF465758 individual amplified regions from different speciemen.
| S-EPMC3847043 | biostudies-literature
Project description:Transcriptome of Sphaeroforma arctica
| PRJEB23831 | ENA