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Tomato leaf curl New Delhi virus (ToLCNDV)

ABSTRACT: Tomato leaf curl New Delhi virus (ToLCNDV) ssDNA plant begomovirus (Family geminiviridae) isolated from Potato in Pakistan.

PROVIDER: PRJEB11738 | ENA |

REPOSITORIES: ENA

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Detection of ToLCNDV resistance in cucumber through agroinoculation under controlled condition

Project description:In this study, Agrobacterium tumefaciens containing recombinant plasmids were used to agroinoculate cucumber seedlings with tandem repeats of DNA-A genome of Tomato leaf curl New Delhi virus (ToLCNDV). The inoculated seedlings, representing both resistant and susceptible accessions, were monitored for symptoms over a period of four weeks. Symptom development was observed in susceptible genotypes, while the resistant genotype remained symptomless. Leaf samples were collected from both agroinoculated and control plants for further analysis. This research sheds light on the pathogenicity of ToLCNDV in cucumber and highlights the potential resistance mechanisms in certain accessions.

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