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The genome of Hypsibius dujardini

ABSTRACT: The genome of Hypsibius dujardini

PROVIDER: PRJEB11910 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1147177_1.fastq.gz	Fastqsanger.gz
	ERR1147177_2.fastq.gz	Fastqsanger.gz
	ERR1147178_1.fastq.gz	Fastqsanger.gz
	ERR1147178_2.fastq.gz	Fastqsanger.gz

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Hypsibius dujardini

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RNA-Seq analysis of developmental, juvenile, and anhydrobiotic stages of Hypsibius dujardini and Ramazzottius varieornatus

Project description:Limno-terrestrial tardigrades enter a state called anhydrobiosis when exposed to desiccation, and acquire tolerance against various extreme environments. The anhydrobiotic tardigrade Hypsibius dujardini, is a non-pigmented tardigrade easy to culture and RNAi method have been established, therefore making it a model tardigrade for tardigrade molecular research. Previous genome assemblies of this tardigrade had increased size due to heterozygosity. Here, we have sequenced the genome of H. dujardini using single individual Illumina DNA-Seq data and PacBio long read data, and employed a heterozygosity aware assembly method to assemble a near-complete high quality genome. In order to annotate the genome with gene predictions, we conducted RNA-Sequencing of various stages of developmental, juvenile, adult, and anhydrobiotic stage H. dujardini and Ramazzottius varieornatus, a tardigrade capable of rapid anhydrobiosis entry, and used these data for gene prediction with BRAKER v1.9 or differential gene expression analysis of the active and anhydrobiotic stages.

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Hypsibius dujardini strain:Sciento

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Hypsibius dujardini strain:Z151

Project description:Hypsibius dujardini strain:Z151 Genome sequencing and assembly

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Hypsibius dujardini strain:Z151

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Project description:Hypsibius dujardinii Transcriptome

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Genome sequencing of a single tardigrade Hypsibius dujardini individual.

Project description:Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.

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