TFIIB ChIP-seq for S. cerevisiae - Genome-wide generalized additive models
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB12260 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1198086.fastq.gz | Fastqsanger.gz | |||
| ERR1198087.fastq.gz | Fastqsanger.gz |
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Project description:ChIP-seq experiment (IP and input control) for the general transcription factor TFIIB in S. cerevisiae
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Project description:Experimental and bioinformatic studies of transcription initiation by RNA polymerase II (RNAP2) have revealed a mechanism of RNAP2 transcription initiation less uniform across gene promoters than initially thought. However, the general transcription factor TFIIB is presumed to be universally required for RNAP2 transcription initiation. Based on bioinformatic analysis of data, TFIIB knockdown in primary and transformed cell lines, and in vitro transcription experiments, we report that TFIIB is dispensable for transcription of most human promoters, but is essential for HSV-1 gene transcription and replication. We report a novel cell cycle TFIIB regulation and involvement of the acetylated TFIIB variant in chromosomal condensation. Taken together, these results establish a new paradigm for TFIIB functionality as a determinant of the human transcriptome, which when downregulated has potent anti-viral effects.
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