Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BiTS Chromatin Immunoprecipitation of H3K4me3 and H3K27ac in Drosophila melanogaster differentiating cardiomyocytes

ABSTRACT: BiTS Chromatin Immunoprecipitation of H3K4me3 and H3K27ac in Drosophila melanogaster differentiating cardiomyocytes

PROVIDER: PRJEB13585 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1361150.fastq.gz	Fastqsanger.gz
	ERR1361146.fastq.gz	Fastqsanger.gz
	ERR1361147.fastq.gz	Fastqsanger.gz
	ERR1361148.fastq.gz	Fastqsanger.gz
	ERR1361149.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

BiTS Chromatin Immunoprecipitation of H3K4me3 and H3K27ac in Drosophila melanogaster differentiating cardiomyocytes

Project description:Here we improved BiTS-ChIP (Bonn et al, Nature Protocols 7, 978-994 (2012)) to identify active enhancer and promoter elements genome wide in the 104 cardiomyocytes that constitute the Drosophila heart tube and represents only ~0.5% of the total cell content of the embryo. A transgenic Drosophila strain expressing nuclear GFP under the control of a cardiac specific enhancer (TinC*>GFP) was used for staged embryo collections at stages 13-14 (10-13h of development). After embryo fixation and dissociation, intact fixed nuclei were fluorescent labelling. Purification of this rare nuclear population was achieved by a two-step sorting procedure, yielding ~98% purity. Chromatin was extracted and used for immunoprecipitation and sequencing (ChIP-seq) to analyze chromatin modifications at promoters (H3K4me3 and H3K27ac) and enhancers (H3K27ac). Two independent biological replicates (from FACS sorting, chromatin preparations and ChIP-Seq) were performed for each mark and sequenced using Illumina HiSeq.

2017-01-10 | E-MTAB-4584 | biostudies-arrayexpress

Chromatin Immunoprecipitation Sequencing of histone modifications (H3K4me3 and H3K27ac) in mouse macrophages (RAW264.7) infected with Mycobacterium tuberculosis

Project description:Histone modifications plays vital role in the regulation of immune response following Mycobacterium tuberculosis infection. Here, mouse macrophages (RAW264.7) were infected with M. tuberculosis (H37Rv) or left uninfected, followed by Chromatin immunoprecipitation with anti-H3K4me3 or anti-H3K27ac antibody and high throughput sequencing.

2025-09-01 | GSE290166 | GEO

H3K4me3 and H3K27ac ChIp-seq

Project description:H3K4me3 and H3K27ac ChIp-seq

| PRJNA512869 | ENA

H3K27ac, H3K4me3 ChIPseq of A549 (T/T) and A549 (C/C) cells

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications, H3K27ac, H3K4me3 in A549 (T/T) and A549 (C/C) cells;

2023-05-10 | GSE225332 | GEO

H3K4me3, H3K27ac, EZH2 in K562

Project description:ChIP-seq of K562 cells: H3K4me3, H3K27ac, EZH2

| PRJEB72776 | ENA

Genome-wide analysis of histone modifications H3K27ac, H3K4me3, and H3K4me1 in differentiating primary human epidermal keratinocytes (NHEK)

Project description:The epidermis is a stratified squamous epithelium that serves to protect the body from dehydration, absorption of chemicals, and invasion of pathogens. In the epidermis, cells of the basal layer contain proliferative potential. As they divide and move upward in the tissue, they progressively differentiate, and activate the gene expression program required to create a barrier; eventually, cells in the uppermost layer are sloughed off from the surface of the epidermis. This system requires continual replacement of differentiating cells from the basal layer. Such a process requires very precise regulation of gene expression to balance proliferation and differentiation and maintain a functioning epithelium. Epigenetic mechanisms of gene regulation, including DNA methylation and histone modification have been shown to play a major role in tissue development and wound healing. To identify regulatory regions in differentiating epidermal cells, we performed ChIP-Seq with antibodies to H3K4me1, and H3K4me3 at 24 hours after induction of differentiation to complement publicly available ENCODE data for these marks in undifferentiated NHEK. With this data, we were able to identify enhancers, defined by the presence of high levels of H3K4me1 and H3K27ac, and low levels of H3K4me3. We identified approximately 20,000 of these regions each in undifferentiated and differentiated keratinocytes. Approximately 20 percent of these regions were shared between the two conditions, while about 30 and 43 percent were unique to differentiated and undifferentiated keratinocytes, respectively.

2016-04-24 | GSE68257 | GEO

H3K27ac, H3K4me3, H3K27me3 and H3K9me3 ChIPseq of H460 (T/T) and H460 (C/C) cells

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications, H3K27ac, H3K4me3, H3K27me3 and H3K9me3 in H460 (T/T) and H460 (C/C) cells

2023-05-10 | GSE213197 | GEO

Chromatin Immunoprecipitation Sequencing of histone modifications (H3K4me3 and H3K27ac) in mouse macrophages (RAW264.7) infected with Mycobacterium tuberculosis

Project description:Chromatin Immunoprecipitation Sequencing of histone modifications (H3K4me3 and H3K27ac) in mouse macrophages (RAW264.7) infected with Mycobacterium tuberculosis

| PRJNA1226502 | ENA

ChIP-seq analysis of histone H3K4me3, H3K27Ac and RNA polymerase II (RNApol II) in HuH-7 cells.

Project description:The RNA-seq profile of the novel LIN28B variant suggested an alternative transcription initiation (ATI) site approximately 20 kb upstream of LIN28B-WT. Chromatin immunoprecipitation sequencing (ChIP-seq) revealed significant enrichment of histone H3K4me3, H3K27Ac and RNA polymerase II (RNApol II) near the ATI site.

2018-01-25 | GSE109575 | GEO

Skeletal muscle myonuclei-specific ChIP-Seq of H3K4me3 and H3K27ac

Project description:Skeletal muscle is a heterogeneous tissue. To study the myo-specific epigenetic landscape in the slow soleus and fast extensor digitorum longus (EDL) muscle, myonuclei were purified with PCM1 -an endogenous mark specific for mature myonuclei. Chromatin immunoprecipitation sequencing (ChIP-Seq) was performed using antibodies against the histone marks H3K4me3 and H3K27ac. Comparison of the differently enriched areas between the two muscles shows that the epigenetic landscape reflects the functional properties of the muscles, each with a distinct regulatory program involving distal regulatory enhancers.

2021-10-22 | GSE182667 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data