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Burkholderia pseudomallei TraDis

ABSTRACT: Identification of Burkholderia pseudomallei essential genes using TraDIS

PROVIDER: PRJEB13678 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1370570.fastq.gz	Fastqsanger.gz
	ERR1370580.fastq.gz	Fastqsanger.gz
	ERR1370571.fastq.gz	Fastqsanger.gz
	ERR1370581.fastq.gz	Fastqsanger.gz
	ERR1370572.fastq.gz	Fastqsanger.gz

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The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei

Project description:Bacterial transcriptomes are dynamic, context-specific and condition-dependent. Infection by the soil bacterium, Burkholderia pseudomallei, causes melioidosis, an often fatal infectious disease of humans and animals. Possessing a large multi-chromosomal genome, B. pseudomallei is able to persist and survive in a multitude of environments. To obtain a comprehensive overview of B. pseudomallei expressed transcripts, we initiated whole-genome transcriptome profiling covering a broad spectrum of conditions and exposures – a so-called “condition compendium”. Using the compendium, we confirmed many previously-annotated genes and operons, and also identified hundreds of novel transcripts including anti-sense transcripts and non-coding RNAs. By systematically examining genes exhibiting highly similar expression patterns, we ascribed putative functions to previously uncharacterized genes, and identified novel regulatory elements controlling these expression patterns. We also used the compendium to elucidate candidate virulence pathways associated with quorum-sensing and infection in mice. Our study showcases the power of a B. pseudomallei condition compendium as a valuable resource for understanding microbial physiology and the pathogenesis of melioidosis.

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Toxicogenomic Effect of Burkholderia pseudomallei in a Human Macrophage Cell (THP-1) Model of Acute Melioidosis Using Whole-genome Microarray

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