Project description:The circular genome sequence of the chemolithoautotrophic euryarchaeon Methanothermobacter marburgensis, with 1,639,135 bp, was determined and compared with that of Methanothermobacter thermautotrophicus. The genomes of the two model methanogens differ substantially in protein coding sequences, in insertion sequence (IS)-like elements, and in clustered regularly interspaced short palindromic repeats (CRISPR) loci.
Project description:To investigate how Methanothermobacter marburgensis adapts to nickel limitation, we performed transcriptomic profiling under 50 nM and 5000 nM nickel conditions. RNA-seq revealed differential expression of hydrogenase-related genes, suggesting a shift in methanogenic electron flow under nickel limitation, with increased reliance on [Fe]-hydrogenase and associated pathways.