Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: RAD-seq of 4608 progeny from a cross of S. cerevisiae strains FY4 and Σ1278b as pilot for BEST genotyping and automated tetrad method.

PROVIDER: PRJEB1744 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR251930.fastq.gz	Fastqsanger.gz
	ERR251931.fastq.gz	Fastqsanger.gz
	ERR251932.fastq.gz	Fastqsanger.gz
	ERR251933.fastq.gz	Fastqsanger.gz
	ERR251934.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 4608

Similar Datasets

Project description:RAD-seq of 390 progeny from a cross of S. cerevisiae strains FY4 and Σ1278b as pilot for BEST genotyping and automated tetrad method.

| PRJEB1580 | ENA

Project description:RAD-seq of 384 progeny from a cross of S. cerevisiae strains S288c and YPS163 as pilot for BEST genotyping and automated tetrad method.

| PRJEB1581 | ENA

RAD methods paper - Threespine Stickleback Lateral Plate Mapping

Project description:Restriction site Associated DNA (RAD) tags are a genome-wide representation of every site of a particular restriction enzyme by short DNA tags. Most organisms segregate large numbers of DNA sequence polymorphisms that disrupt restriction sites, which allow RAD tags to serve as genetic markers spread at a high-density throughout the genome. Here, we demonstrate the applicability of RAD markers for both individual and bulk-segregant genotyping. First, we show that these markers can be identified and typed on pre-existing microarray formats. Second, we present a method that uses RAD marker DNA to rapidly produce a low-cost microarray genotyping resource that can be used to efficiently identify and type thousands of RAD markers. We demonstrate the utility of the former approach by using a tiling path array for the fruit fly to map a recombination breakpoint, and the latter approach by creating and utilizing an enriched RAD marker array for the threespine stickleback. The high number of RAD markers enabled localization of a previously identified region, as well as a second novel region also associated with the lateral plate phenotype. Taken together, our results demonstrate that RAD markers, and the method to develop a RAD marker microarray resource, allow high-throughput, high-resolution genotyping in both model and non-model systems. Keywords: microarray genotyping

2007-01-24 | GSE5773 | GEO

S288c_x_YJM145

Project description:Array hybridization data for 611 yeast segregants to determine genotype at each marker. DNA from each segregant was digested with DNAseI, labeled with Cy5-ddATP, and hybridized individually to a custom agilent 8x15k, genotyping array . Note: Tetrad refers to the tetrad from the cross of YJM145 x S288c that each segregant was dissected from. Tag ID refers to unique yeast barcode has been integrated at the ho locus in a segregant.

2012-06-29 | E-MTAB-724 | biostudies-arrayexpress

YCR13u

Project description:RAD-seq genotyping of progeny from a cross between strains YO795 (EC-33) and YO796 (YPS163).

| PRJEB7334 | ENA

Yeast spores genotyping to analyze the fate of meiotic DNA double strand breaks

Project description:Yeast spores genotyping to analyze the fate of meiotic DNA double strand breaks. For control crossovers are recirpocal events based on the information from two independent spore hybridizations from a unique tetrad were used. Genotyping procedure followed as described in Bourgon et al 2009

2012-01-01 | E-MTAB-508 | biostudies-arrayexpress

YCR13s

Project description:RAD-seq genotyping of galactose-tolerant progeny from a cross between strains YO795 (EC-33) and YO796 (YPS163)

| PRJEB7336 | ENA

Mar1 deletion and RNA enrichment in Cryptococcus neoformans: pilot data for a high-throughput sequencing course

Project description:Mar1 deletion and RNA enrichment in Cryptococcus neoformans: pilot data for a high-throughput sequencing course. The goal of this project was to generate pilot data in preparation for a summer course on high-throughput sequencing where participants prepared their own RNA-Seq libraries and analyzed the resulting data. This pilot experiment addressed two questions: 1. Does this experimental system (Cryptococcus neoformans H99 wildtype and mar1 deletion mutant grown in YPD and tissue culture media) provide a good dataset for course participants to analyze. 2. Which rRNA depletion method is best to use in the wetlab component of the course. This data was generated in preparation for the intensive summer course on high-throughput sequencing, funded by NIH grant 5R25EB023928-03 "A hands-on, integrative next-generation sequencing course: design, experiment, and analysis".

2021-02-22 | GSE160397 | GEO

WGS of a serie of Arabidopsis thaliana tetrad's products to study the role of RECQ4A, RECQ4B, FIGL1 and FANCM genes in the rate, nature and distribution of meiotic recombination products (CO and NCO)

Project description:In this study, two accessions of Arabidopsis thaliana (Columbia and Landsberg erecta) were crossed and tetrads were obtained using thanks to the the quartet mutation, which keeps the 4 haploid pollen grains from a single meiosis attached together. By fertilising a plant (of ecotype Columbia) with a single tetrad, then selecting seeds from siliques containing exactly four seeds and finally sequencing the 4 developed plants, we can access the complete history of meiotic recombination events occurring in a single male meiosis. Comparison of the genomic sequences (WGS) of the 4 plants in a tetrad makes it possible to identify meiotic COs and NCOS events in each tetrad thanks to the numerous polymorphisms specific to each of the parental genomes. The analysis of the WGS tetrads data consists of genotyping a series of SNV markers (differentiating Columbia and Landsberg) positioned on the five chromosomes for the 4 (M1, M2, M3, M4) individuals of a tetrad, representing the 4 chromatids of each chromosomes. A total of 20 tetrads, the F1 and the two parental accessions Columbia and Landsberg erecta were sequenced.

2024-12-02 | E-MTAB-14435 | biostudies-arrayexpress

Project description:RAD-seq of progeny from cross UC5 x DBVPG1853 for ploidy estimation

| PRJEB1767 | ENA