Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of ionizing radiation on A549, H460 and H1299 lung cancer cells

ABSTRACT: Effect on gene expression after 4 days of a single, 15 Gy dose of ionizing X ray radiation on A549, H460 and H1299 human lung cancer cell-lines

PROVIDER: PRJEB18075 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1740330_1.fastq.gz	Fastqsanger.gz
	ERR1740330_2.fastq.gz	Fastqsanger.gz
	ERR1740340_1.fastq.gz	Fastqsanger.gz
	ERR1740340_2.fastq.gz	Fastqsanger.gz
	ERR1740331_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Expression data from H1299 and H460 lung cancer cell lines

Project description:To identify a set of genes related to radioresistance, we analyzed the time-series gene expression profiles of radioresistant H1299 and radiosensitive H460 lung cancer cells in response to 2 Gy of ionizing radiation (IR) by performing quadratic regression (QR) analysis. Out of the 21,331 genes, we selected 6,538 genes by QR analysis from the gene expression profile of H460 cells and 6,086 genes from that of H1299 cells. Most of the genes identified in the H460 cells were classified into continuously up- or down-regulated groups, while the major QR groups were transiently changed groups in the H1299 cell line. From gene ontology analysis of the major QR groups, the DNA damage response was commonly enriched in both cell lines. DNA repair-related genes such as ATM, ATR, TP53BP1, BRCA1, MRE11, NBN and RAD50 were particularly up-regulated in H1299 cells. Suppression of these DNA repair-related genes using siRNA made H1299 cells radiosensitive to ionizing radiation. The data suggest that differential responses to DNA damage confer radioresistance to cancer cells, and provide potential novel targets for sensitizing radiotherapy.

2010-02-27 | GSE20549 | GEO

Translatome of A549, H1299 and HBE cells

Project description:We performed translatome and transcriptome sequencing of A549, H1299 and HBE cells and analyzed the translation ratio (TR) of all genes Total RNA and ribosome-bound RNA were extracted from A549, H1299 and HBE cells, and the polyA+ mRNA was sequenced using Illumina GAIIx and HiSeq-2000. The reads were mapped to RefSeq RNA reference sequences and the expression level were quantified by rpkM calculation.

2013-03-05 | E-GEOD-42006 | biostudies-arrayexpress

Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on microRNA expression in the NCI-H460 human lung large cell carcinoma cell line

Project description:To understand the effect of nicotine on sensitivity of cancer cells to radiation or anti-cancer drugs, NCI-H460 human lung large cell carcinoma cells were treated with 6 Gy ionizing radiation or 1 uM cisplatin after exposure to 0.5 uM nicotine or cigarette smoke extract. MicroRNA expression in total RNA extracted from the treated cells was quantified using the 7th generation miRCURY™ locked nucleic acid microarray platform from Exiqon® (Vedbaek, Denmark).

2014-12-31 | GSE42333 | GEO

Effect of cigarette smoke extract, cisplatin, nicotine and/or ionizing radiation on gene expression in the NCI-H460 human lung large cell carcinoma cell line

Project description:To understand the effect of nicotine on sensitivity of cancer cells to radiation or anti-cancer drugs, NCI-H460 human lung large cell carcinoma cells were treated with 6 Gy ionizing radiation or 1 uM cisplatin after exposure to 5 uM nicotine or cigarette smoke extract. Whole genome expression in total RNA extracted from the treated cells was quantified using the GeneChip™ Human Gene 1.0 ST microarray platform from Affymetrix® (Santa Clara, CA).

2014-12-31 | GSE42172 | GEO

Translatome of A549, H1299 and HBE cells

Project description:We performed translatome and transcriptome sequencing of A549, H1299 and HBE cells and analyzed the translation ratio (TR) of all genes

2013-03-05 | GSE42006 | GEO

RNA-sequencing in irradiated and normal A549 cells.

Project description:The aim of this study is to investigate the role of circRNAs/miRNAs/lncRNAs in radioresistance in A549 cells. For irradiation, A549 cells were exposed to a single fraction 2 GY or 4GY X-ray dose using an X-RAD 160-225 instrument (Precision X-Ray, Inc., Branford, CT, USA; filter: 2 mm AI; 42 cm, 225 kV/s, 12.4 mA, 2.0 Gy/min). The irradiated A549 cells (after a single dose of irradiation) and normal A549 cells were cultured for further 48h and then collected its total RNA for high-throughput sequencing.

2018-12-27 | GSE124396 | GEO

Gene expression in drug-resistant and circulating tumor cell cultured H460 or A549 cells

Project description:Gene expression in drug resistant and circulating tumor cell cultured H460 or A549 cells

2020-09-29 | GSE158640 | GEO

HIF1A ChIP-seq from HCT116, RKO, A549, and H460 cells in normoxia and hypoxia

Project description:ChIP-seq analysis was used to identify HIF1A binding sites in normoxia and hypoxia in HCT116, RKO, A549, and H460 cells

2021-01-29 | GSE145157 | GEO

RNA sequencing showing the different response pattern between lung epithelial cells and lung cancer cells after radiation

Project description:To seek if ionizing radiation have different biological effect on lung normal cells and cancer cells, we treated lung epithelial cell line BEAS-2B, non-small cell lung cancer cell line A549 and small cell lung cancer cell line H446 with 10 Gy X-ray radiation

2022-12-31 | GSE211118 | GEO

Coordinated regulation of MAPK signaling pathway and microRNA in response to ionizing radiation in lung cancer cells

Project description:microRNA regulates cellular responses to ionizing radiation (IR) through the translational control of target genes. We analyzed time-series changes in microRNA expressions upon γ-irradiation in H1299 lung cancer cell lines using microarray. Significantly changed microRNAs were selected based on ANOVA analysis, target genes of which were enriched to MAPK signaling pathway. Concurrent analysis of mRNA and microRNA uncovered that the expression of miR-26b and its target ATF2 mRNA were inversely correlated in γ-irradiated H1299 cells. The overexpression of miR-26b induced the suppression of ATF2 in γ-irradiated cells. When we inhibit the MAPK signaling pathway using SP600125, JNK inhibitor, the expression of miR-26b was induced even in γ-irradiated H1299 cells. From these results, we concluded that the expression of miR-26b was coordinated regulated by MAPK signaling pathway upon ionizing radiation, and MAPK signaling pathway was regulated by miR-26b in turn. We analyzed the time-series miRNA profiles of radioresistant H1299 cells in response to 2 Gy of ionizing radiation (IR) by performing quadratic regression (QR) analysis to identify genes associated with radioresistance

2011-09-13 | E-GEOD-30075 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data