ABSTRACT: Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Frisson nitrogen-fixing nodules (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Finale nitrogen-fixing nodules (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Sprint-2 nitrogen-fixing nodules (3 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed NGB1238 nodulated roots (4 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Analysis of transcriptomic profile of pea (Pisum sativum L.) breed Sparkle nodulated roots (2 week after inoculation) by Massive Analysis of cDNA Ends (MACE).
Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.
Project description:This study aims to compare the potential of standard RNA-sequencing (RNA-Seq) and 3’ massive analysis of c-DNA ends (MACE) RNA-sequencing for the analysis of fresh tissue and describes transcriptome profiling of formalin-fixed paraffin-embedded (FFPE) archival human samples by MACE.
Project description:The secretion of metabolites by plant roots is a key determinant of microbial growth and colonisation. We have used Pisum sativum and its natural symbiont Rhizobium leguminosarum (it can form N2 fixing nodules on pea roots) to study the natural metabolites secreted by roots. To do this root secretion was harvested from pea plants grown under sterile conditions. This root exudate was then concentrated and used as a sole carbon and nitrogen source for growth of the bacteria in the laboratory. These bacteria were harvested in mid-exponential growth and RNA extracted for microarray analysis. As control cultures the bacteria were grown on 30 mM pyruvate as a carbon source and 10 mM ammonium chloride as a nitrogen source and RNA extracted. Two colour microarrays were performed using root exudate cultures versus pyruvate ammonia grown cultures. This was done in biological triplicate.