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Circularized chromosome conformation capture (4C-seq) in A549 and U2OS cells stably expressing rat GRα

ABSTRACT: Circularized chromosome conformation capture (4C-seq) in A549 and U2OS cells stably expressing rat GRα

PROVIDER: PRJEB18677 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1762140_1.fastq.gz	Fastqsanger.gz
	ERR1762140_2.fastq.gz	Fastqsanger.gz
	ERR1762141_1.fastq.gz	Fastqsanger.gz
	ERR1762141_2.fastq.gz	Fastqsanger.gz
	ERR1762142_1.fastq.gz	Fastqsanger.gz

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Circularized chromosome conformation capture (4C-seq) in A549 and U2OS cells stably expressing rat GR

Project description:To investigate the interaction landscape at the GILZ locus we performed 4C-seq in A549 (ATCC CCL-185) and U2OS cells stably expressing rat GR (Rogatsky et al. , Mol Cell Biol, 1997. 17(6): p. 3181-93.) upon hormone treatment (1.5 h, 1 M dexamethasone). Experiments were performed in two biological replicates using hg19 chrX:106,960,488-106,960,865 as a viewpoint.

2018-04-12 | E-MTAB-5350 | biostudies-arrayexpress

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Project description:circularized chromosome conformation capture followed by sequencing (4C-seq) in the heterozygous (T/C) A549 cell line

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Project description:Mouse Bcell, upon ectopic expression of the transcription factor Cebpa for 18h, can be reprogrammed to iPS with extremely high efficiency. To understand the molecular control of this phenomena we performed multiple high throughtput functionnal genomic analysis. Circularized chromosome conformation capture assay (4C) taking as view point Klf4 promoter and a putative enhancer situated a -100kb upstream

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