Project description:UM1 and UM2 are paired cell lines generated from a oral cancer patient [Nakayama, et al Invas. Metast., 18 (1998), pp. 219–228]. UM1 is highly invasive and exhibits elevated metastatic potential as compared to UM2.
Project description:By performing PRMT5 knockdown in the human tongue squamous cell carcinoma cell line UM1, we analyzed the effects of this treatment on the global gene transcription level and its impact on the genomic binding capacity of the transcription factor ΔNp63α. Using genomic binding data supplemented with the histone activation marker H3K27ac, we identified a significant impact of PRMT5 on the transcriptional regulatory capability of ΔNp63α.
Project description:High glucose has a significant effect on cancer progression. We aim to investigate the effect of glucose on oral cancer cells at gene expression level. We use microarray to identify differentially expressed gene in oral cancer cells (UM1) that cultured in different glucose concentration.
