Salmonella Typhimurium Genotypic Characterization in Rio de Janeiro State, Brazil
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ABSTRACT: Pathogenicity and antibiotic resistance genes profile among Salmonella Typhimurium isolated from Chicken and swine carcasses in two distinct geographical regions from Rio de Janeiro State, Brazil
Project description:Database of raw 16s rRNA sequences from an ultra-small bacterial community able to degrade microcystin from Jacarepaguá Lagoon, Rio de Janeiro, Brazil
Project description:Rio Grande do Sul is Brazil’s leading wine-producing state and hosts the majority of the country’s Geographical Indications. This study hypothesizes that commercial Merlot and Chardonnay wines from different Geographical Indications and municipalities of Rio Grande do Sul exhibit distinct chemical fingerprints identifiable by untargeted UHPLC–MS/MS metabolomic profiling. To test this hypothesis, a strategy based on ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC–MS/MS) was employed. The integration of metabolomic profiling with multivariate statistical analyses, alongside the evaluation of reducing and antioxidant capacity, enabled a differentiation of wines from the two main mesoregions, Campanha and Serra Gaúcha, despite the high variability of the dataset. Metabolite annotation revealed that several some discriminant compounds were associated with agronomic practices, oenological processes, and climatic conditions, with particular emphasis on variations in ultraviolet radiation exposure. Nitrogen-containing metabolites, such as amino acids, peptides, and choline, as well as flavonols and anthocyanins, consistently played a central role in regional discrimination. Notably, the recurrent detection of quercetin as a distinguishing feature of Serra Gaúcha wines underscores its potential utility as a chemical marker linked to terroir. Collectively, these findings demonstrate that metabolomic profiling constitutes a robust and reliable approach for investigating geographical origin, typicity, and authenticity in Brazilian wines.
Project description:The established cell lines RT4, 5637 and T24 from human bladder TCC were obtained from the Cell Bank of the Federal University of Rio de Janeiro, Brazil. The 5637 cells harbor two TP53 mutations, at codon 72 (Arg . Pro) and codon 280 (Arg . Thr).T24 cells contain a TP53 allele encoding an in-frame deletion of tyrosine 126. Both cell lines were established from high-grade bladder tumors. No specific mutations were detected in RT4 cells, which had been established from a low-grade papillary bladder tumor. The RT4 and T24 cell lines were maintained in DulbeccoM-^Rs modified EagleM-^Rs medium (Sigma-Aldrich, Inc, St Louis, MO, USA), and the 5637 cells were kept in Roswell Park Memorial Institute medium (Sigma-Aldrich). Both media were supplemented with 10% fetal bovine serum (Cultilab Ltd, Campinas, Brazil), 100 U/mL penicillin G (Sigma-Aldrich), 100 U/mL streptomycin (Sigma-Aldrich) and 1% kanamycin sulfate (Amresco, Branded Products Group, Solon, OH, USA); cells were cultured at 37M-:C in an atmosphere of 5% CO2. A pooled reference design was chosen. More specifically, each array was hybridized with the same reference sample labeled with Cy5, while the experimental samples (control or treated) were labeled with Cy3.
Project description:We report a pilot investigation for poly-A RNAs differentially expressed during Mycobacterium tuberculosis infection. Participation in this investigation from March 2010 to July 2013 was voluntary, only subjects that were >18 years old and that informed written consent were considered eligible. The recruitment of tuberculosis (TB) patients was done at public hospitals in Rio de Janeiro, Brazil. The diagnostic criteria for active pulmonary tuberculosis was at least one AFB (acid-fast bacilli) -positive sputum sample for M. tuberculosis and/or positive sputum culture and/or compatible clinical evolution for pulmonary TB and less than 15 days of anti-TB treatment and was in accordance with those of the Brazilian Ministry of Health. Blood was collected from recent close contacts (rCt) and active tuberculosis (TB) index cases (n=6). Latent TB infection (LTBI) was accessed by both tuberculin skin test (TST, cut-off = 5mm) and in house interferon-gamma release assays (IGRA, cut-off = 100 pg/ml), therefore, 12 rCt were classified as uninfected controls and 16 with LTBI. Subsequently, the sequencing was performed following the standard protocols on Illumina HiSeq® 2500 Sequencing System (Illumina, San Diego, CA) running 100 bp paired-end reads (PE100) and generating approximately 30 million reads passing filter for each sample to produce the mRNA reads. Mining these RNAseq data, highly prominent modulation of DOCK9, EPHA4, and NPC2 mRNA expression was observed in the TB samples, indicating that they might have a role in TB pathogenesis. These differential modulations upon M. Tuberculosis infection were further validated by additional evidences in larger cohorts from different geographical areas.