Project description:The bacterial strains P. ruminicola ATCC 19189, M. elsdenii DSM 20460, B. fibrisolvens DSM 3071, E. hallii DSM 3353, R. intestinalis DSM 14610, M. formatexigens DSM 14469 and F. prausnitzii DSM 17677 were cultivated as single cultures, in pairwise cultures and full consortia. Bacteria were grown anaerobically in hungate tubes containing YCFA medium, supplemented with 0.5% wheat straw fiber or fructose as carbon sources.
Project description:Colonization with Paleniella intestinalis bypasses genetic resistance, consistently72 inducing arthritis incidence in non-susceptible C57BL/6 mice; P. intestinalis activates colonic CD11b + CD11c+ myeloid cells, driving systemic Th1774 cell differentiation; In vitro, outer membrane vesicles from P. intestinalis or RA-derived S. copri RPC0176 primed DCs that drive Th17 differentiation in an IL-6-dependent manner;Transfer of P. intestinalis-primed DCs or its outer membrane vesicles alone increases78 arthritis incidence in non-susceptible C57BL/6 mice
Project description:We have performed strand-specific RNA-seq of trophozoites from four different Giardia intestinalis strains (A=WB and AS175, B=GS, E=P15). Comparison of mRNA levels in four different Giardia intestinalis strains.
Project description:To investigate the transcriptional responses of intestinal epithelial cells and Giardia intestinalis, assemblage A isolate WB-C6, trophozoites during infection, we infected human enteroids with preconditioned trophozoites for 1h and 3h. Giardia intestinalis trophozoites were preconditioned before the infection with either DMEM/F-12 or DMEM/F-12 supplemented with 10% FBS to modify the trophozoites’ fitness.
Project description:We report the comprehensive sequencing of small RNA libraries created from different developmental stages (larva and gastrula) of the basal chordate, Ciona intestinalis. These libraries were used for the identification of microRNAs in this organism.
