Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H

ABSTRACT: ChIP-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H

PROVIDER: PRJEB20314 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR1916490.fastq.gz	Fastqsanger.gz
	ERR1916491.fastq.gz	Fastqsanger.gz
	ERR1916484.fastq.gz	Fastqsanger.gz
	ERR1916485.fastq.gz	Fastqsanger.gz
	ERR1916486.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

RNA-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H.

Project description:RNA-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H.

| PRJEB20313 | ENA

RNA-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H.

Project description:This experiment aimed to determine the genes that were upregulated when mutant p53 (R273H) was expressed. We used H1299 lung cancer cells that are endogenously p53-null and overexpressed the p53 mutant.

2017-04-16 | E-MTAB-5652 | biostudies-arrayexpress

ChIP-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H

Project description:This experiment was performed to determine which gene promoters mutant p53 binds and transcriptionally regulates in order to understand how mutant p53 accomplishes its gain of function phenotype.

2017-04-16 | E-MTAB-5653 | biostudies-arrayexpress

Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

Project description:The expression of the forkhead transcription factor CHES1, also known as FOXN3, is reduced in many types of cancers. In vitro, CHES1 expression suppresses cell proliferation in tumor cell lines but not in normal cells. Conversely shRNA-mediated depletion of CHES1 increases tumor cell proliferation. We used microarrays to characterize the gene expression changes induced by CHES1 in H1299 cancer cells H1299 cells were infected with a retroviral vector that expresses CHES1 or an empty vector. After 5 days of infection, RNA was extracted from three independent replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.

2013-08-01 | E-GEOD-49422 | biostudies-arrayexpress

RNA-seq of MCF10A cells stably expressing empty vector, GFP, miR-105, or MYC

Project description:To identify gene expression changes associated with overexpression of miR-105 or MYC in MCF10A non-cancerous human mammary epithelial cells, we analyzed RNA isolated from engineered MCF10A cell lines that stably express empty vector, GFP, miR-105, or MYC by RNA-seq. Gene expression in cells overexpressing miR-105 or MYC was compared to cells expressing the empty vector or GFP, both of which served as controls in this experiment.

2018-02-11 | GSE106502 | GEO

Microarray analysis of A673 cell line stably transfected with empty vector pc3.1 versus A673 cell lines stably transfected with pcDNA3.1-LMO3-BORCS5

Project description:In order to understand what genes were regulated by the presence of a new fusion transcript (LMO3-BORCS5), we stably transfected either an empty vector (pc3.1) or vector containing LMO3-BORCS5 sequence into A673 human Ewing sarcoma cell line. 2 different clones were obtained for each condition (4 conditions total), and 4 different RNA extractions were performed for each clone (4 x 4 = 16 conditions total).

2019-06-30 | E-MTAB-7021 | biostudies-arrayexpress

Expression profile of HEK293 cells stably transfected with PRL-1 (PTP4A1) or empty pcDNA4 vector (control)

Project description:The multifunctional protein tyrosine phosphatase PRL-1 (Gene Symbol: PTP4A1) has been identified as an important oncogene with roles in promoting cell proliferation, survival, migration, invasion, and metastasis. However, little is currently known about the signaling pathways through which it mediates its effects. We used Affymetrix Human Genome U133 Plus 2.0 arrays to examine the global molecular changes of gene expression occurring in response to stable PRL-1 overexpression in human embryonic kidney 293 (HEK293) cells. Total RNA was extracted from triplicate cultures of HEK293 cells stably overexpressing PRL-1 (HEK293-PRL-1) and HEK293 cells stably transfected with empty pcDNA4 vector (HEK293-Vector). Samples were hybridized to Affymetrix HG U133 Plus 2.0 microarrays (1 array per sample for a total of 6 arrays). One of the arrays hybridized with a PRL-1 transfected sample was excluded after qRT-PCR demonstrated that this sample did not exhibit increased PRL-1 expression over the level of the empty vector controls. The remaining 2 HEK293-PRL-1 samples both expressed PRL-1 at least 2-fold higher than any of the 3 empty vector controls.

2013-07-30 | E-GEOD-42586 | biostudies-arrayexpress

Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

2013-08-01 | GSE49422 | GEO

NCI-H1299 cell line transfected with human DENND2D and vector

Project description:Gene expression for NCI-H1299 cell line transfected with human DENND2D and vector (pcDNA3.1/V5-His TOPO TA vector) respectively.

2010-05-04 | GSE16890 | GEO

NCI-H1299 cell line transfected with human DENND2D and vector

Project description:Gene expression for NCI-H1299 cell line transfected with human DENND2D and vector (pcDNA3.1/V5-His TOPO TA vector) respectively. The microarray experiment was designed to perform four replicates for each H1299-DENND2D and H1299-vector sample. cRNA used in replication 1 and 2 was from the same label reaction to perform the hybridization replicates.

2010-05-27 | E-GEOD-16890 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data