Project description:Escherichia coli DH1 cultures with treated with 6% 1,4 Butanediol for 1 hour and compared with untreated cultures The data from this experiment was used to identify a candidate for further study as described in Szmidt et al 2013 Utilizing a highly responsive gene, yhjX, in E. coli based production of 1,4-Butanediol submitted to Chemical Engineering Science 4x72k E.coli gene expression microarrays were used to study the genes that are differentialy expressed in the strain DH1 grown in defined medoin and exposed to 6% 1,4 Butanediol for one hour at mid-log growth stage.
Project description:Escherichia coli DH1 cultures with treated with 6% 1,4 Butanediol for 1 hour and compared with untreated cultures The data from this experiment was used to identify a candidate for further study as described in Szmidt et al 2013 Utilizing a highly responsive gene, yhjX, in E. coli based production of 1,4-Butanediol submitted to Chemical Engineering Science
Project description:(S)-1,3-butanediol [(S)-1,3-BDO] is a chiral diol and stereoisomer of (R)-1,3-BDO with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (S)-1,3-BDO and (S)-3-hydroxybutyrate [(S)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Differential gene expression analysis was performed to identify genes specifically induced during (S)-1,3-BDO and (S)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.
Project description:(R)-1,3-butanediol [(R)-1,3-BDO] is a chiral diol with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (R)-1,3-BDO and (R)-3-hydroxybutyrate [(R)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Gluconate was used as a reference carbon source. Differential gene expression analysis was performed to identify genes specifically induced during (R)-1,3-BDO and (R)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.
Project description:<p>Small volatile molecules are considered to play a role in promoting plant growth, with acetoin and 2,3-butanediol being representative examples. In this study, we employed metabolic engineering of Bacillus subtilis to enhance the production of acetoin and 2,3-butanediol, and investigated their effects on the rhizosphere microbiome and metabolic processes of vegetables, aiming to elucidate the mechanisms by which the acetoin and 2,3-butanediol complex promotes plant growth. First, high-yield B. subtilis strains capable of producing acetoin and 2,3-butanediol were obtained through screening and mutagenesis from soil isolates. Starting from these strains, further metabolic engineering strategies were implemented, including the deletion of by-product synthesis pathways, overexpression of biosynthetic genes, and optimization of the TCA cycle. These modifications significantly improved the production levels of acetoin and 2,3-butanediol. </p>
