Small RNA and mRNA-seq of Arabidopsis thaliana WT and hyl1-2 seedlings in light or dark.
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB20420 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1937940.fastq.gz | Fastqsanger.gz | |||
| ERR1937950.fastq.gz | Fastqsanger.gz | |||
| ERR1937941.fastq.gz | Fastqsanger.gz | |||
| ERR1937951.fastq.gz | Fastqsanger.gz | |||
| ERR1937942.fastq.gz | Fastqsanger.gz |
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Project description:Red light can affect a variety of responses in Arabidopsis. We characterize the early gene expression patterns of seedlings exposed to 1 hour of red light using a small sized sample of 5, 7-day-old seedlings and also performed dark controls. Methods that were developed for tissue extraction and labeling for microarrays were tested using these samples Experiment Overall Design: Only 5, 7 day-old dark-grown Arabidopsis seedlings were treated as described and harvested for RNA extraction and hybridization on Affymetrix microarrays. We pooled samples from one Petri plate with MS medium 2% sucrose. Treatment was 1 hour of red light 630 nm (max) and dark controls
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