Project description:This study compares gene expression in three strains (CPL2H1, CPL2H1 gtf1/gtf1 and CPL2H1 otf1/otf1) with the genetic background of Candida parapsilosis CLIB 214 (CBS 604). Total RNA was isolated using hot acid phenol extraction. A TruSeq stranded mRNA library was sequenced on an Illumina NovaSeq 6000 system.
Project description:Candida parapsilosis CLIB 214 (CBS 604) was grown on synthetic media containing hydroxyaromatic compounds as a sole carbon source. The cells cultivated in medium containing galactose were used as a control. Total RNA was isolated using hot acid phenol extraction. A TruSeq stranded mRNA library was sequenced on an Illumina NovaSeq 6000 system.
Project description:Non-conventional yeasts represent a great genetic and phenotypic diversity with potential for industrial strain development in the bio-production of green chemicals. In recent years, mass genome sequencing of non-conventional yeasts has opened avenues to improved understanding of transcriptional networks and phenotypic plasticity and gene function, including the discovery of novel genes. Here, we investigated the gene expression changes at low-pH in three strains of the acidophilic yeast Maudiozyma bulderi (previously Kazachstania bulderi): CBS8638, CBS8639 and NRRL-Y27205. The comparison of the transcriptome of cells growing in a bioreactor at pH=5.5 vs pH= 2.5, primarily showed dysregulation of genes involved in cell wall integrity, with NRRL-Y27205, the least acidophilic strain, showing the largest transcriptional response when compared to the other two strains. We identified four uncharacterised genes, unique to M. bulderi ,and predicted function as transporters, upregulated at low pH. We also showed that M. bulderi cell wall and membrane lipid composition is not significantly affected by low pH unlike Saccharomyces cerevisiae. Overall, our data on transcriptional variability in M. bulderi highlights genes and cellular pathways involved in the acidophilic adaptation of this species and can aid further strain development.
