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Single Bacterial Cell Whole Genome Amplification

ABSTRACT: Single Bacterial Cell Whole Genome Amplification

PROVIDER: PRJEB2063 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR022126_1.fastq.gz	Fastqsanger.gz
	ERR022126_2.fastq.gz	Fastqsanger.gz
	ERR022127_1.fastq.gz	Fastqsanger.gz
	ERR022127_2.fastq.gz	Fastqsanger.gz
	ERR022128_1.fastq.gz	Fastqsanger.gz

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Project description:Advances in biochemical technologies have led to a boost in the field of single cell genomics. Observation of the genome at a single cell resolution is currently achieved by pre-amplification using whole genome amplification (WGA) techniques that differ by their biochemical aspects and as a result by biased amplification of the original molecule. Several comparisons between commercially available single cell dedicated WGA kits (scWGA) were performed, however, these comparisons are costly, were only performed on selected scWGA kit and more notably, are limited by the number of analyzed cells, making them limited for reproducibility analysis. We benchmarked an economical assay to compare all commercially available scWGA kits that is based on targeted sequencing of thousands of genomic regions, including highly mutable genomic regions (microsatellites), from a large cohort of human single cells (125 cells in total). Using this approach, we could analyze the genome coverage, the reproducibility of genome coverage and the error rate of each kit. Our experimental design provides an affordable and reliable comparative assay that simulates a real single cell experiment. Results demonstrate the need for a dedicated kit selection depending on the desired single cell assay.

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