Project description:Human coronary artery endothelial cells were infected with Chlamydophila pneumoniae. We monitor cellular gene expression profiling altered by life cycle of Chlamydophila pneumoniae using Affymetrix Human Genome U133 Plus 2.0 Array.
Project description:Experimental screening of a compound library identified a molecule that potently inhibits the growth of the obligate intracellular bacterial pathogen Chlamydia trachomatis in human cells. To identify the molecular target of the compound, three mutant bacterial strains resistant to its inhibitory action were generated by long-term passage in the presence of initially low but increasing concentrations of the molecule. Subsequently, genomic DNA of the three mutant and the wildtype bacteria was isolated and subjected to whole genome sequencing to identify resistance-promoting mutations.
Project description:Human coronary artery endothelial cells were infected with Chlamydophila pneumoniae. We monitor cellular gene expression profiling altered by life cycle of Chlamydophila pneumoniae using Affymetrix Human Genome U133 Plus 2.0 Array. 5 time points were measured, 5min, 25min, 2h, 24h, 60h after infection