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Transcription profiling of wild type and XY Nrg1 knock out male pooled embryonic gonads to decipher the role of this gene in sexual differentiation

ABSTRACT: Transcription profiling of wild type and XY Nrg1 knock out male pooled embryonic gonads to decipher the role of this gene in sexual differentiation

PROVIDER: PRJEB21056 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1993311.fastq.gz	Fastqsanger.gz
	ERR1993312.fastq.gz	Fastqsanger.gz
	ERR1993313.fastq.gz	Fastqsanger.gz
	ERR1993314.fastq.gz	Fastqsanger.gz
	ERR1993315.fastq.gz	Fastqsanger.gz

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Transcription profiling of wild type and XY Nrg1 knock out male pooled embryonic gonads to decipher the role of this gene in sexual differentiation

Project description:In mammals, sex determination depends on the paternal transmission of the Y chromosome, which bears the Sry gene. At the time of sex determination, Sry becomes up-regulated in XY gonads initiating the expression of various factors required for testicular development. Most of these factors remain to be identified. As a consequence, the genetic causes of gonadal dysgenesis in human patients, other than those involving SRY, remain unknown. We found that Nrg1 is a new gene involved in early testicular development. To study the role of Nrg1 in embryonic testicular differentiation, conditional inactivation of Nrg1 was achieved with the help of a Wt1-Cre line. Mutant mice show defects of testis development. In order to correlate the defects of cellular processes and the misregulated gene network(s), expression profiles were examined in controls and mutants testes at 13.5 dpc. Each testis sample was pooled from eight embryos.

2017-10-02 | E-MTAB-5772 | biostudies-arrayexpress

Transcription profiling of XX and XY sorted mouse somatic support cells and XX and XY mouse whole gonads to identify potential new mammalian gonadal sex determining genes.

Project description:Gonadal sex determining (GSD) genes that initiate fetal ovarian and testicular development and differentiation are expressed in the cells of the urogenital ridge that differentiate as somatic support cells (SSCs), i.e., granulosa cells of the ovary and Sertoli cells of the testis. To identify potential new mammalian GSD genes, we analyzed the gene expression differences between XX and XY SSCs cells isolated from the gonads of embryonic day (E) 13 mouse fetuses carrying an EGFP reporter transgene expressed specifically in SSCs. In addition, genome wide expression differences between XX and XY E13 whole gonads were examined. Newly identified differentially expressed transcripts are potential GSD genes involved in unexplained human sex reversal cases. Experiment Overall Design: Two seperate RNA samples were obtained from E13 XX and XY sorted EGFP+ cells, and two seperate RNA samples were obtained from E13 XX and XY pooled gonads. Approximately 20ng of total RNA from each sample was used to generate biotin-labelled cDNA. Approximately 2.5ug biotin labelled cDNA of each sample was used for each Mouse Genome 430v2.0 GeneChip array (Affymetrix). Significantly differentially expressed transcripts were identified using R/maanova. Statistical significance was determined at a false discovery rate value of equal to or less than 1%.

2007-11-30 | E-GEOD-4928 | biostudies-arrayexpress

gonads gene expression profiling in WT and Sox9flox/flox; Sf1:creTr/+ mice

Project description:In mammals, gonadal differentiation is the first step of sex determination, and the transcription factor Sox9 promotes testis differentiation. Here we used the XY Sox9flox/flox; Sf1:creTr/+ mouse model and show that the lack of Sox9 expression induces a full sex reversal of E13.5 XY Sox9flox/flox; Sf1:creTr/+ gonads compared to XY Sox9flox/flox. Keywords: gonads gene expression profiling in WT and Sox9flox/flox; Sf1:creTr/+ mice

2011-11-01 | GSE21650 | GEO

Expression changes in the XY fetal gonads due to iron deficiency

Project description:Expression changes in the XY fetal gonads due to iron deficiency

| PRJNA1212543 | ENA

Strain differences in e11.5 XY gonad transcriptome suggest a mechanism for the sensitivity of C57BL/6J to sex reversal

Project description:Transcriptional profiling of individual mouse embryonic (e11.5) XY gonads comparing inbred strains that are sensitive (C57BL/6J) and resistant (129S1/SvImJ) to XY sex reversal, and their reciprocal F1 hybrids.

2009-10-30 | GSE16917 | GEO

gonads gene expression profiling in WT and Sox9flox/flox; Sf1:creTr/+ mice

2011-11-01 | E-GEOD-21650 | biostudies-arrayexpress

Expression analysis of Wv/Wv fetal gonads

Project description:Following sex determination, XY and XX gonads develop into a testis and an ovary, respectively. Depending on the sex of the gonad, resident germ cells will subsequently be committed to either spermatogenesis or oogenesis. In this study we took advantage of the Wv/Wv mouse genetic model, in which gonads are almost devoided of germ cells, to uncover gene expression underlying fetal germ cell development.

2010-12-20 | GSE23908 | GEO

Germ cell sexual differentiation requires Nanos2-mediated repression of Dazl in mice [Set 2]

Project description:In mouse embryos, sex-specific differentiation of primordial germ cells (PGCs) begins with distinct cell cycle regulations: while PGCs in female (XX) gonads enter meiosis, PGCs in male (XY) gonads defer meiosis and arrest cell cycle1,2. The mitotic quiescence in XY gonads requires Nanos2, an evolutionarily conserved RNA-binding protein implicated in RNA degradation3-5; however, underlying mechanisms including the identity of its target mRNAs in vivo remain unknown. Here we show that Nanos2 negatively regulates Dazl (Deleted in azoospermia-like), a germline-specific gene encoding an RNA-binding protein that is implicated in translational control and meiotic initiation6,7. Comprehensive microarray analyses identified Dazl as one of the genes that were up-regulated in Nanos2-deficient XY gonads, but down-regulated in Nanos2-expressing XX gonads. Analysis of a BAC transgenic mouse line, in which the 3’UTR of Dazl can be removed by Flp-Frt recombination system, showed that repression of Dazl is likely achieved by the association of Nanos2 with Dazl’s 3’UTR. Elevated levels of Dazl expression in the transgenic XY PGCs resulted in abnormal progression of cell cycles including meiosis, reminiscent of the phenotype observed in Nanos2-/- XY gonads. This phenotype was suppressed by inhibiting retinoic acid (RA) signaling, suggesting that Nanos2 represses Dazl to prevent RA-induced cell cycle progression in XY PGCs. Furthermore, we show that a part of Nanos2-associated mRNAs, most of which are up-regulated in Nanos2-/- XY gonads, can also associate with Dazl, and that excess Dazl impedes localization of Nanos2 to processing-bodies where RNA degradation takes place, suggesting that repression of Dazl is required for Nanos2 to efficiently degrade its target mRNAs. These data provide the first link between the two RNA-binding proteins that play pivotal roles in sexual differentiation of murine PGCs.

2015-12-01 | GSE56794 | GEO

Germ cell sexual differentiation requires Nanos2-mediated repression of Dazl in mice [Set 1]

2015-12-01 | GSE56793 | GEO

Identification of Nrg1 enhancer binding proteins

Project description:Hyperglycemia is a risk factor for breast cancer-related morbidity and mortality. Hyperglycemia induces Neuregulin 1 (Nrg1) overexpression in breast cancer, which subsequently promotes tumor progression. However, molecular mechanisms underlying hyperglycemia-induced Nrg1 overexpression remain poorly understood. Here, We used DNA-protein pull-down using Nrg1 enhancer sequence as bait followed by LC/MS and identified RBPJ as a key component of the Nrg1 enhanceosome. We show that hyperglycemia causes active histone modifications at the Nrg1 enhancer, forming enhanceosome complexes where RBPJ, P300, and SETD1A are recruited to upregulate Nrg1 expression.

2023-01-29 | PXD038668 | Pride

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