Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA-seq of mouse adipose stem and progenitor cells

ABSTRACT: scRNA-seq of mouse adipose stem and progenitor cells

PROVIDER: PRJEB21183 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2000050.fastq.gz	Fastqsanger.gz
	ERR2000060.fastq.gz	Fastqsanger.gz
	ERR2000070.fastq.gz	Fastqsanger.gz
	ERR2000080.fastq.gz	Fastqsanger.gz
	ERR2000090.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 288

Similar Datasets

scRNA-seq of mouse adipose stem and progenitor cells

Project description:We analysed a well-established fraction of mouse subcutaneous adipose-derived SVF cells that is generally considered to harbour adipose stem and progenitor cells (ASPCs). To study the molecular characteristics and the subpopulation structure of ASPCs, we performed three replicate scRNA-seq experiments. We collected Lin- (CD31- CD45- TER119-) CD29+ CD34+ SCA1+ cells from the mouse subcutaneous SVF of transgenic mice, in which red fluorescent protein (RFP) is induced in Dlk1-expressing cells upon feeding with tamoxifen. While CD29, CD34, and SCA1 are generally expected to enrich for stem cells, DLK1 has previously been suggested to specifically mark pre-adipocytes. The submission includes the raw data of all sequenced cells, while we only processed 208 high quality single cells. RFP status is indicated in the processed files.

2018-05-01 | E-MTAB-5802 | biostudies-arrayexpress

RNA-seq of mouse adipose stem and progenitor cells

Project description:Population control for the scRNA-seq based analysis a well-established fraction of mouse subcutaneous adipose-derived stromal vascular fraction (SVF) cells that is generally considered to harbour adipogenic stem and progenitor cells (ASPCs). We collected Lin- (CD31- CD45- TER119-) CD29+ CD34+ SCA1+ cells from the mouse subcutaneous SVF of transgenic mice, in which red fluorescent protein (RFP) is induced in Dlk1-expressing cells upon feeding with tamoxifen. While CD29, CD34, and SCA1 are generally expected to enrich for stem cells, DLK1 has previously been suggested to specifically mark pre-adipocytes.

2018-05-01 | E-MTAB-5818 | biostudies-arrayexpress

RNA-seq of mouse adipose stem and progenitor cells

Project description:RNA-seq of mouse adipose stem and progenitor cells

| PRJEB21572 | ENA

3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

Project description:3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

| PRJEB21182 | ENA

3' RNA-seq of CD142+/- mouse adipose stem and progenitor cells

Project description:To characterize the newly discovered Areg cells, we sampled at least four replicates of mouse adipose stem and progenitor cells (ASPCs), CD142+ ASPCs and CD142- ASPCs at four distinct time-points: (1) immediately post-sort (D0), (2) after attachment (5 hours after plating, 5H), (3) after culturing (24 hours after plating, D1) and (4) after adipogenic differentiation (day 12 after plating, day 8 after induction of differentiation, D12), and investigated their transcriptome through a highly multiplexed 3 polyA-selective mRNA-sequencing assay.

2018-05-01 | E-MTAB-5787 | biostudies-arrayexpress

scRNA-seq of Mus musculus subcutaneous Lin- cells derived from the subcutaneous adipose stromal vascular fraction

Project description:We used the resolving power of single-cell transcriptional profiling to molecularly characterize the mouse adipose stem and progenitor cell-enriched, subcutaneous adipose stromal vascular fraction. We molecularly assessed CD45- CD31- SVF cells using the 10x Genomics Chromium (10x) platform.

2019-05-24 | E-MTAB-6677 | biostudies-arrayexpress

Multilayered Omics Revealsl Sex- and Depot-Dependent Adipose Progenitor Cell Heterogeneity

Project description:White adipose tissue (WAT) harbors functionally diverse subpopulations of adipose progenitor cells that differentially impact tissue plasticity in a sex- and depot-dependent manner. To date, the molecular basis of this cellular heterogeneity has not been fully defined. Here, we describe a multilayered omics approach to dissect adipose progenitor cell heterogeneity from in three dimensions: progenitor subpopulation, sex, and anatomical localization. We applied state-of-the-art mass spectrometry methods to quantify 4870 proteins in eight different stromal cell populations from perigonadal and inguinal WAT of male and female mice and acquired transcript expression levels of 15477 genes using RNA-seq. Notably, our data highlight the molecular signatures defining sex differences in PDGFR+ preadipocyte differentiation and identify regulatory pathways that functionally distinguish adipose tissue PDGFRb+ subpopulations. The data are freely accessible as a resource at "Pread Profiler. Together, the multilayered omics analysis provides unprecedented insights into adipose stromal cell heterogeneity.

2023-03-11 | PXD023829 | Pride

Identification of a mesenchymal progenitor cell hierarchy in adipose tissue (scRNA-seq)

Project description:Identification of a mesenchymal progenitor cell hierarchy in adipose tissue (scRNA-seq)

| PRJNA532356 | ENA

Identification of a mesenchymal progenitor cell hierarchy in adipose tissue (scRNA-seq)

Project description:The healthy growth of adipose tissue depends on the capacity of progenitor cells to undergo denovo adipogenesis. However, the cellular hierarchy and mechanisms governing adipocyteprogenitor differentiation are incompletely understood. Here, we identify a lineage hierarchy25 consisting of distinct mesenchymal cell types present in mouse and human adipose tissue. Cellsmarked by Dpp4 expression are highly proliferative, multipotent progenitors that give rise toIcam1+ committed pre-adipocytes and a related adipogenic population marked by Clec11a andCd142 expression. TGFβ maintains DPP4+ cell identity and inhibits adipogenic commitment ofDPP4+ and CD142+ cells. Intriguingly, DPP4+ progenitors reside in the reticular interstitium that30 envelope many organs including adipose depots. Altogether, this study defines the adipose lineagehierarchy and identifies a new anatomical niche for multipotent mesenchymal progenitors.

2019-05-01 | GSE128890 | GEO

3' RNA-seq of CD142+/- human adipose stem and progenitor cells

Project description:3' RNA-seq of CD142+/- human adipose stem and progenitor cells

| PRJEB21181 | ENA