Project description:To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing.
Project description:We reported an atlas of de novo-defined, full-length macaque gene models on the basis of single molecule long-read transcriptome sequencing (Iso-seq).