Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spy 94a

ABSTRACT: Spy 94a nuclear DNA sequences

PROVIDER: PRJEB21883 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2039574.fastq.gz	Fastqsanger.gz
	ERR2039575.fastq.gz	Fastqsanger.gz
	ERR2039576.fastq.gz	Fastqsanger.gz
	ERR2039577.fastq.gz	Fastqsanger.gz
	ERR2039578.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

Expression data from wildtype and spy-3 roots

2015-05-16 | GSE50952 | GEO

Expression data from wildtype and spy-3 roots

Project description:SPY is an O-linked N-acetylglucosamine transferase involved in many signaling and developmental processes in Arabidopsis. SPY also has a role in root development. In the spy mutant, a middle cortex is produced prematurely. To understand how SPY regulates root cortex proliferation, we sought to identify the genes affected by the spy mutation in the root. The transciptomes from the root of one-week-old seedlings, Col or spy-3 mutant, were compared. The seedlings were grown on a nylon membrane placed on 1X MS agar medium and the plates are positionally vertically in a Pecival growth chamber with 16 hr light and 8 hr dark. Three biological replicates were performed.

2015-05-16 | E-GEOD-50952 | biostudies-arrayexpress

SPY-3_OX_dehydration

Project description:Both wild-type and SPY-3 OX A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in GM plates for 3 weeks under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22M-0C. Dehydration treatment: The 3-week-old plants were grown for 2 h without watering.

2012-08-24 | E-MEXP-3362 | biostudies-arrayexpress

Dictyostelium discoideum O-fucome from WT, SPY-OE, and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Ser and O-Thr fucosylated proteins from WT, SPY-OE and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser and anti O-Thr fucose polyclonal antibodies in 3 Technical Replicates, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation), and SPY_OE (mutant, SPY overexpressor strain).

2025-01-30 | PXD057018 | Pride

mtDNA sequences from Spy 572a scapula and Fonds-de-Foret femur

Project description:mtDNA sequences from Spy 572a scapula and Fonds-de-Foret femur

| PRJEB39136 | ENA

Rhizobium deserti strain:SPY-1

Project description:Rhizobium deserti strain:SPY-1 Genome sequencing and assembly

| PRJNA526777 | ENA

SPY mediates O-fucose modification of hundreds of proteins and sugar-dependent growth in Arabidopsis_(Part 1: HCD)

Project description:The recent discovery of SPY-catalyzed protein O-fucosylation reveals a novel mechanism for regulating nucleocytoplasmic protein functions in plants. Genetic evidence indicates important roles of SPY in diverse developmental and physiological processes, however, the upstream signal controlling SPY activity and the downstream substrate proteins O-fucosylated by SPY remain largely unknown. Here, we demonstrate that SPY mediates sugar-dependent growth. We further identify hundreds of O-fucosylated proteins using lectin affinity chromatography followed by mass spectrometry. All the O-fucosylation events quantified in proteomic analyses are undetectable or decreased in the spy mutants and thus likely catalyzed by SPY. The O-fucosylome includes mostly nuclear and cytosolic proteins. Many O-fucosylated proteins function in essential cellular processes, hormone signaling, and developmental programs, consistent with the genetic functions of SPY. The O-fucosylome also includes many proteins modified by O-linked-N-acetylglucosamine (O-GlcNAc) and by phosphorylation downstream of the target of rapamycin (TOR) kinase, revealing the convergence of these nutrient signaling pathways on key regulatory functions such as post-transcriptional/translational regulation and hormonal responses. Our study identifies numerous targets of SPY/O-fucosylation and potential nodes of crosstalk among sugar/nutrient signaling pathways, enabling future dissection of the signaling network that mediates sugar regulation of plant growth and development.

2023-05-10 | PXD038490 | Pride

SPY mediates O-fucose modification of hundreds of proteins and sugar-dependent growth in Arabidopsis_(Part 2: EtHCD)

2023-05-10 | PXD038491 | Pride

Dictyostelium discoideum O-Thr-fucome from WT and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Thr-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Thr fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056857 | Pride

Dictyostelium discoideum O-Ser-fucome from WT and SPY-KO vegetative cells

Project description:Dictyostelium discoideum O-Ser-fucosylated proteins from WT and SPY-KO vegetative cells were immunoprecipitated using anti O-Ser fucose polyclonal antibodies in 3 Biological Replicates, with 3 to 6 Technical Replicates each, by comparing AX3 (WT strain) against SPY-KO (mutant, deficient in O-fucosylation).

2025-01-30 | PXD056853 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data