Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spikes

ABSTRACT: spikes

PROVIDER: PRJEB22043 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2072190_1.fastq.gz	Fastqsanger.gz
	ERR2072190_2.fastq.gz	Fastqsanger.gz
	ERR2072200_1.fastq.gz	Fastqsanger.gz
	ERR2072200_2.fastq.gz	Fastqsanger.gz
	ERR2072210_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 302

Similar Datasets

Transcriptional reference map of hormone responses in wheat spikes

Project description:Phytohormones are key regulators of plant growth, development, and signalling networks involved in responses to diverse biotic and abiotic stresses. Transcriptional reference maps of hormone responses have been reported for several model plant species such as Arabidopsis thaliana, Oryza sativa, and Brachypodium distachyon. However, because of species differences and the complexity of the wheat genome, these transcriptome data are not appropriate reference material for wheat studies. We comprehensively analysed the transcriptomic responses in wheat spikes to seven phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA), ethylene (ET), cytokinin (CK), salicylic acid (SA), and methyl jasmonic acid (MeJA). A total of 3386 genes were differentially expressed at 24 h after the hormone treatments. Furthermore, 22.7% of these genes exhibited overlapping transcriptional responses for at least two hormones, implying there is crosstalk among phytohormones. We subsequently identified genes with expression levels that were significantly and differentially induced by a specific phytohormone (i.e., hormone-specific responses). The data for these hormone-responsive genes were then compared with the transcriptome data for wheat spikes exposed to biotic (Fusarium head blight) and abiotic (water deficit) stresses. Our data were used to develop a transcriptional reference map of hormone responses in wheat spikes.

2017-09-05 | GSE103430 | GEO

Single-cell RNA sequencing with molecular spikes to determine RNA counting accuracy

Project description:Molecule counting is central to single-cell sequencing, yet no experimental strategy to evaluate counting performance exist. Here, we introduce RNA spike-ins containing inbuilt unique molecular identifiers (molecular spikes) that we use to monitor single-cell RNA counting performance across methods and to identify experimental steps essential for accurate counting. In this dataset, we add molecular spikes to popular single-cell RNA-seq protocols: SCRB-seq, Smart-seq3 and 10x Genomics (v2). For SCRB-seq and Smart-seq3, we also include variations of the library preparation procedure that are suspected to lead to changes in the UMI counting accuracy.

2022-02-16 | E-MTAB-10372 | biostudies-arrayexpress

Transcription profiling of specific organs of wheat spikes in response to Fusarium graminearum inoculation

Project description:Organ specific microarray analysis were performed to identify genes responding to Fusarium graminearum inoculation in specific organs of wheat spikes.

2007-04-25 | E-TABM-97 | biostudies-arrayexpress

Macrovascular infiltration and spikes in circulating tumor cells in cancer patients approaching end of life

Project description:End-of-life neoplastic events related to carcinoma lethality are poorly characterized. With the purpose of exploring biological events in cancer patients nearing death, we conducted an observational, prospective, case-control study enrolling 21 patients with solid tumors and 10 patients without known malignancy, complemented by a retrospective validation cohort of 1,250 cancer patients. In our prospective cohort, we observed remarkable spikes in circulating tumor cell (CTC) counts immediately before death (P < 0.0001), as well as pathological evidence of macrovascular infiltration and large-vessel occlusion obtained through rapid autopsy. In the validation cohort, radiological evidence of macrovascular infiltration emerged as the strongest predictor of poor survival – independent of clinical metastasis – in treatment-homogenous patients with colorectal, lung, ovarian, hepatocellular, or pancreatic cancer (hazard ratio range: 4.0 - 22.4). These findings suggest that spikes in CTC formation and consequent macrovascular failure could be pivotal end-of-life events associated with cancer lethality, providing a rationale for future clinical trials aimed at curbing tumor infiltration into large vessels.

2025-07-23 | GSE295441 | GEO

Expression profiling in young spikes of pistillody wheat line compared with normal wheat line

Project description:We have employed whole genome microarray expression profiling to identify genes conferring induction of pistillody, homeotic transformation of stamens into pistil-like structures. As a result, we identified five genes which show higher expression levels in pistillody line compared with normal line. Quantitative expression analysis using real-time PCR indicated that among five genes a calmodulin (CaM)-binding protein gene, WCBP1 (wheat calmodulin-binding protein 1), is obviously up-regulated in the young spikes of the pistillody line. The full-length cDNA sequence for WCBP1 showed it is a member of the ACBP60 family CaM-binding protein. Expression patterns were compared between the pistillody line and normal line. Total RNA samples were isolated from young spikes (3-10mm in length) at floret differentiation stage. Two independent experiments were conducted in each experiments.

2012-12-31 | E-GEOD-37486 | biostudies-arrayexpress

Single-cell sequencing of HEK293FT cells with molecular spikes and recorded cell size

Project description:Individual HEK cells were dispensed using an F.SIGHT into individual wells while recording cell diameters. Each well contained 0.0321 pg of molecular spike-ins, a highly complex set of 264 molecular spikes, based on 11 unique spike sequences spanning different lengths (570 to 3070 nts) and GC contents (40-60%). Libraries were generated with Smart-seq3xpress protocol.

2022-02-16 | E-MTAB-11433 | biostudies-arrayexpress

Expression profiling in young spikes of pistillody wheat line compared with normal wheat line

2012-12-31 | GSE37486 | GEO

Single-cell RNA sequencing with molecular spikes to determine RNA counting accuracy

Project description:Single-cell RNA sequencing with molecular spikes to determine RNA counting accuracy

| PRJEB44662 | ENA

Single-cell sequencing of HEK293FT cells with molecular spikes and recorded cell size

Project description:Single-cell sequencing of HEK293FT cells with molecular spikes and recorded cell size

| PRJEB50824 | ENA

Complement factor C1q mediates sleep spindle disruption and epileptic spikes after mild brain injury

Project description:Complement factor C1q mediates sleep spindle disruption and epileptic spikes after mild brain injury

| PRJNA744171 | ENA