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Single-cell RNA-seq of of dermal fibroblasts using microfluidic droplet capture (stimulated cells)

ABSTRACT: Single-cell RNA-seq of of dermal fibroblasts using microfluidic droplet capture (stimulated cells)

PROVIDER: PRJEB22073 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2078880.fastq.gz	Fastqsanger.gz
	ERR2078890.fastq.gz	Fastqsanger.gz
	ERR2078900.fastq.gz	Fastqsanger.gz
	ERR2078910.fastq.gz	Fastqsanger.gz
	ERR2078920.fastq.gz	Fastqsanger.gz

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Single-cell RNA-seq of of dermal fibroblasts using microfluidic droplet capture (stimulated cells)

Project description:The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.<br>This experiment contains data of dermal fibroblasts from 3 human individuals, stimulated with dsRNA (poly I:C) for 6 hours, sequenced by 10X Genomics technology. Corresponding data from unstimulated cells are found under ArrayExpress accession <a href="/arrayexpress/experiments/E-MTAB-5988">E-MTAB-5988</a>.

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