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The evolutionary impact of Intragenic FliA Promoters

ABSTRACT: The evolutionary impact of Intragenic FliA Promoters

PROVIDER: PRJEB22568 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2116781.fastq.gz	Fastqsanger.gz
	ERR2116782.fastq.gz	Fastqsanger.gz
	ERR2116783.fastq.gz	Fastqsanger.gz
	ERR2116784.fastq.gz	Fastqsanger.gz

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Similar Datasets

The evolutionary impact of Intragenic FliA Promoters

Project description:The evolutionary impact of Intragenic FliA Promoters

| PRJEB22569 | ENA

The evolutionary impact of Intragenic FliA Promoters

Project description:We looked at binding sites in a FliA FLAG-tagged wild-type Salmonella enterica serovar Typhimurium strain.

2017-09-18 | E-MTAB-6049 | biostudies-arrayexpress

The evolutionary impact of Intragenic FliA Promoters

Project description:We compared RNA levels in wild-type Salmonella enterica serovar Typhimurium with RNA levels in a mutant strain in which the fliA gene was deleted.

2017-09-18 | E-MTAB-6048 | biostudies-arrayexpress

The evolutionary impact of intragenic FliA promoters in proteobacteria.

Project description:In Escherichia coli, one sigma factor recognizes the majority of promoters, and six 'alternative' sigma factors recognize specific subsets of promoters. The alternative sigma factor FliA (σ28 ) recognizes promoters upstream of many flagellar genes. We previously showed that most E. coli FliA binding sites are located inside genes. However, it was unclear whether these intragenic binding sites represent active promoters. Here, we construct and assay transcriptional promoter-lacZ fusions for all 52 putative FliA promoters previously identified by ChIP-seq. These experiments, coupled with integrative analysis of published genome-scale transcriptional datasets, strongly suggest that most intragenic FliA binding sites are active promoters that transcribe highly unstable RNAs. Additionally, we show that widespread intragenic FliA-dependent transcription may be a conserved phenomenon, but that specific promoters are not themselves conserved. We conclude that intragenic FliA-dependent promoters and the resulting RNAs are unlikely to have important regulatory functions. Nonetheless, one intragenic FliA promoter is broadly conserved and constrains evolution of the overlapping protein-coding gene. Thus, our data indicate that intragenic regulatory elements can influence bacterial protein evolution and suggest that the impact of intragenic regulatory sequences on genome evolution should be considered more broadly.

| S-EPMC5943157 | biostudies-literature

RNA-seq to investigate the role of FliA-transcribed protein coding genes in Salmonella Typhimurium

Project description:FliA in S. Typhimurium directs transcription of large numbers of unstable, non-coding RNAs from intragenic promoters. Also in this study, we identify two previously unreported FliA-transcribed protein coding genes, including sdiA, which encodes a transcription factor that responds to quorum sensing signals produced by other bacteria.

2020-09-09 | E-MTAB-9504 | biostudies-arrayexpress

ChIP-seq to investigate the role of FliA-transcribed protein coding genes in Salmonella Typhimurium

2020-09-09 | E-MTAB-9503 | biostudies-arrayexpress

Transcription-driven Chromatin Repression of Intragenic Promoters

Project description:Transcription-driven Chromatin Repression of Intragenic Promoters

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Transcription-driven Chromatin Repression of Intragenic Promoters

Project description:The goal of this study is to investigate a chromatin-based mechanism that suppresses intragenic initiation of RNAPII transcription in A.thaliana. We demonstrate that RNAPII transcription across gene promoters represses their function in plants. The FACT histone chaperone complex is required for this repression mechanism by co-transcriptionally enforcing elevated levels of H3K4me1 at repressed intragenic promoters;

2018-04-26 | GSE113677 | GEO

fliA regulon of Helicobacter hepaticus

Project description:Differential gene regulation in Helicobacter hepaticus between wild type bacteria and fliA mutant

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V1 vs V1 FliA knockout

Project description:Competive hybridization of V1 variant of genome sequenced C. jejuni strain 11168 versus V1 variant in which fliA gene encoding sigma 28 has been disrupted Keywords: repeat sample

2004-03-03 | GSE521 | GEO

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