Project description:The yeast Rhodotorula mucilaginosa shown the capability to degrade patulin by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin patulin was analyzed. The molecular mechanism of Rhodotorula mucilaginosa withstand patulin was revealed.
Project description:Constipation affects approximately 15% of the global population, and gut microbiota dysbiosis is implicated in its pathogenesis. Rothia mucilaginosa, a commensal bacterium with established anti-inflammatory properties, has not been previously investigated for its effects on intestinal function. In this study, we evaluated the therapeutic potential of R. mucilaginosa in a loperamide-induced constipation mouse model using multiomics approaches. Twenty-six SPF male C57BL/6 mice were divided into normal control (NC, n=8), constipation model control (MC, n=8), and R. mucilaginosa-treated (RG, n=10) groups. R. mucilaginosa intervention significantly improved fecal output and induced gut microbiota remodeling, including enrichment of Akkermansia muciniphila and Alistipes finegoldii. To characterize host molecular responses, RNA-seq was performed on colon tissues to identify differentially expressed genes and pathways associated with constipation alleviation, with particular focus on neuroactive pathway activation.
Project description:Purpose: The goal of this study is to looked at the expression levels of defense genes in orange fruit in response to the application of the antagonist R. mucilaginosa enhanced with SA Methods: Three identical and evenly distributed wounds (5 mm diameter and 3 mm deep) made at the equator of each fruit were inoculated with 30 µL cell suspension of R. mucilaginosa (1×108 cells/mL) + 0.2 mM SA, whiles cell suspension of R. mucilaginosa alone (1×108 cells/mL) was used as a control. Treated fruits were then kept in plastic baskets and covered with film wraps to maintain relative humidity of 95% at storage temperature of 20 ºC. After 72 h of storage the wound tissues were excise and immediately kept in liquid nitrogen and maintained at -80 ºC for the analysis of the RNA. There were three replications per each sample. Results: The results showed that 46 genes were identified as DEGs. Among these 34 were up-regulated and 12 were down regulated, 10 of the RNA-seq these were validated using qRT-PCR. RNA-seq data had a linear relationship withqRT_PCR with a corelation coefficent value of (R2) 0.8388. Conclusion: Our finding showed that the defense genes of orange fruit were up-regulated by the innoculation of R. mucilaginosa enhanced with 0.2 mM SA in the fruit.
