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Cappable seq of Photorhabdus luminescens TTO1

ABSTRACT: Cappable seq of Photorhabdus luminescens TTO1

PROVIDER: PRJEB23380 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2192824.fastq.gz	Fastqsanger.gz
	ERR2192825.fastq.gz	Fastqsanger.gz
	ERR2192826.fastq.gz	Fastqsanger.gz
	ERR2192827.fastq.gz	Fastqsanger.gz
	ERR2192828.fastq.gz	Fastqsanger.gz

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Project description:Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. In the entomopathogenic bacterium Photorhabdus luminescens, a dam ortholog was identified. Overexpression of dam in P. luminescens did not impair growth ability in vitro. In contrast, compared to a control strain harboring an empty plasmid, a significant decrease in motility was observed in the dam-overexpressing strain. In addition, the dam-overexpressing P. luminescens strain showed a delayed virulence compared to that of the control strain after injection in larvae of the lepidopteran Spodoptera littoralis. These results reveal that Dam plays a major role during P. luminescens insect infection.

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